补虚化瘀祛痰饮对老年冠心病稳定性心绞痛患者抗氧化作用的影响

作者	单位
闫凌云	河南省鹤壁市职业技术学院医学院
张玉泉	河南省鹤壁市职业技术学院医学院
王喜梅	河南省鹤壁市职业技术学院医学院
张新萍	河南省鹤壁市职业技术学院医学院
李稀科	河南省鹤壁市京立医院

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中文摘要:

目的研究补虚化瘀祛痰饮组方对老年冠心病稳定性心绞痛的抗氧化作用机制。方法选取老年冠心病稳定性心绞痛患者(中医诊断为胸痹心痛证)40例,随机分为中药干预组和对照组,每组20例。测定治疗前后超氧化物歧化酶(SOD)活性、血浆脂质过氧化物(LPO)含量以及外周血单个核细胞锰超氧化物歧化酶(manganese-containing superoxide dismutase,MnSOD)基因mRNA的表达水平。结果对照组治疗前后血浆SOD活性及LPO含量差异无统计学意义(P>0.05),而中药干预组治疗后血浆SOD活性显著升高,LPO含量显著降低(P<0.01);中药干预组MnSOD基因mRNA表达水平显著高于对照组(P<0.01)。结论补虚化瘀祛痰饮能诱导单个核细胞MnSOD基因表达,提高SOD活性,降低LPO含量,从而维持心肌细胞的氧化和抗氧化平衡,阻断脂质过氧化的连锁反应,干预氧自由基的产生并增强消除氧自由基的功能,这些可能是该方剂有效治疗老年冠心病稳定性心绞痛的机制之一。

英文摘要:

Objective To investigate the acting mechanism of Buxu Huayu Qutan Decoction (BHQD) for impacting the anti-oxidative capacity in aged patients with stable angina pectoris of coronary heart disease (AP-CHD). Methods Forty patients of AP-CHD,Chinese medicine diagnosed as Xiong-bi,were equally assigned to the treatment group and the control group. Superoxide dismutase (SOD) activity and lipid peroxide (LPO) contents in blood,and mRNA expression of manganese-containing superoxide dismutase (MnSOD) in peripheral mononuclear cells were determined before and after treatment by biochemical and molecular biologic techniques. Results No significant change of plasma SOD and LPO was found in the control group after treatment (P>0.05),while the plasma SOD activity increased and LPO content lowered in the treatment group significantly (P<0.01). Moreover,mRNA expression of MnSOD in the treatment group after treatment was obviously higher than that in the control group (P<0.01). Conclusions The acting mechanism of BHQD for AP-CHD treatment might partially due to its effects in inducing gene expression of MnSOD in mononuclear cells,enhancing SOD activity,decreasing LPO content,maintaining oxidation/antioxidation equilibrium in myocardial cells,blocking the chain reaction of lipid peroxidation,intervening the production and enhancing the scavenging of oxygen free radicals.

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