| 曹玉雪,董竞成,崔焱,刘宝君,段晓虹,谢谨玉,吴金峰,乐晶晶.慢性阻塞性肺疾病合并哮喘大鼠气道炎症特点及补肾益气中药干预研究[J].中国中西医结合杂志,2009,(8):716-721 |
| 慢性阻塞性肺疾病合并哮喘大鼠气道炎症特点及补肾益气中药干预研究 |
| Feature of Airway Inflammation in Asthmatic Rats Complicated with Chronic Obstructive Pulmonary Disease and Effect of Chinese Herbs for Reinforcing Shen and Supplementing Qi on It |
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| DOI： |
| 中文关键词: 慢性阻塞性肺疾病 支气管哮喘 补肾益气中药 白细胞介素 干扰素-γ 炎症 |
| 英文关键词:chronic obstructive pulmonary disease bronchial asthma Chinese herbs for reinforcing Shen and supplementing qi interleukin interferon-γ inflammation |
| 基金项目:国家重点基础研究发展计划(973计划)资助项目(No.2009CB523000);高等学校博士学科点专项科研基金(No.20060246063) |
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| 中文摘要: |
| 目的观察慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)合并哮喘大鼠模型气道炎症的变化及补肾益气中药对大鼠气道炎症的干预作用。方法84只挪威大鼠随机分为正常对照组、COPD模型组、哮喘模型组、COPD合并哮喘模型组和COPD合并哮喘低、中、高剂量中药组,每组12只。观察(1)大鼠症状;(2)肺组织病理变化;(3)大鼠气道反应性;(4)检测大鼠血清细胞IL-4、干扰素-γ(IFN-γ)、白细胞介素(interleukin,IL)-6、IL-8的变化。结果COPD合并哮喘用药组大鼠症状减轻;COPD合并哮喘模型组病理结果与COPD模型组有相似之处,支气管、肺组织出现以淋巴细胞为主的炎症细胞浸润,纤毛脱落,部分肺泡壁破裂,肺泡腔扩大,但同时伴有明显的嗜酸粒细胞浸润。用药后炎症渗出减轻;COPD合并哮喘模型组与哮喘模型组变化趋势相似(P>0.05),气道阻力随着乙酰甲胆碱(Mch)浓度的增加而加大;与COPD模型组比较,COPD合并哮喘模型组血清IL-4水平升高,INF-γ明显降低(P<0.05);与正常对照组和哮喘模型组比较,COPD合并哮喘模型组血清INF-γ明显降低,差异有统计学意义(P<0.05);补肾益气中药能降低COPD合并哮喘模型组血清IL-4、IL-8水平;COPD合并哮喘模型组随着补肾益气中药剂量的增加,血清IFN-γ有上升的趋势。结论COPD合并哮喘大鼠存在IL-4明显升高,INF-γ的明显降低,与哮喘Th1/Th2失衡的机理相似。补肾益气中药可改善COPD合并哮喘大鼠症状,抑制气道炎症,考虑可能与改善Th1/Th2失衡等机制有关。 |
| 英文摘要: |
| Objective To observe the airway inflammatory change in asthmatic rats complicated with chronic obstructive pulmonary disease (COPD) and to assess the intervention effects of Chinese herbs for reinforcing Shen and supplementing qi (CH) on it. Methods Eighty-four Norway rats were randomized into 7 groups,the normal control group (A),the COPD model group (B),the asthma model group (C),the combined COPD and the asthma model group (D),and the three CH treated groups (E,F and G,combined model rats administered by low-,moderate-and high-dose CH,respectively),12 rats in each group. Changes of symptoms,pathologic changes of the lung tissue,airway reactivity,and serum levels of interleukin-4,interleukin-6,interleukin-8 (IL-4,IL-6,and IL-8) and interferon-γ (IFN-γ) in rats were observed. Results Symptoms were alleviated in the three CH treated groups. Similar pathological features were shown in group B and D,showing inflammatory cell,mainly lymphocyte,infiltration in bronchial and lung tissues,with cilia denudation,partial alveolar wall rupture,alveolar cavity expansion,and accompanied with evident eosinophilic infiltration. These inflammatory exudation in group E-G was alleviated,while in group C,it developed showing a trend similar to that in group D. Airway resistance raised along with the concentration of Mch used. In group D,the serum level of IL-4 was higher than that in group B,and level of INF-γ was lower than that in group A,B and C (all P<0.05). CH showed a lowering effect on serum levels of IL-4 and -8,and a dose-dependent rising effect on IFN-γ. Conclusions IL-4 significantly increased and INF-γ decreased in rat model of combined COPD and asthma,its mechanism is similar to that of Th1/Th2 imbalance in asthma. Chinese herbs for reinforcing Shen and supplementing qi could improve the symptoms and inhibit the airway inflammation in the combined COPD and asthma model rats,its mechanism might be related with the alleviation of TH1/TH2 imbalance. |
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