洁泽1号凝胶对小鼠阴道内乳杆菌、糖原及乳铁蛋白的影响

作者	单位
陈琢	华中科技大学同济医学院附属同济医院中西医结合科
高文	华中科技大学同济医学院附属同济医院中西医结合科
马永贵	华中科技大学附属同济医院药学部
陆付耳	华中科技大学同济医学院附属同济医院中西医结合科
黄光英	华中科技大学同济医学院附属同济医院中西医结合科

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中文摘要:

目的观察中药复方洁泽1号凝胶对小鼠阴道内乳杆菌、糖原及乳铁蛋白的作用,评价洁泽1号对阴道内环境的影响。方法取雌性昆明小鼠100只,随机分5组,每组20只。即空白对照组、空白凝胶组(基质对照组)、洁泽1号凝胶高(2g/mL)、中(1g/mL)、低(0.5g/mL)剂量组,每只小鼠皮下注射生理剂量的苯甲酸雌二醇(E2)2μg,72h后予小鼠阴道分别注入相应的药物凝胶20μL,并分别于注射药物凝胶后24h及72h阴道灌洗液培养,进行乳杆菌活菌计数,并检测阴道组织糖原含量及乳铁蛋白表达。结果各浓度药物组与空白对照组在乳杆菌计数、糖原含量及乳铁蛋白表达方面比较差异均无统计学意义(P>0.05),洁泽1号凝胶高、中、低剂量组在24h与72h比较,差异亦无统计学意义(P>0.05)。结论一次性使用洁泽1号凝胶对小鼠阴道内乳杆菌、糖原含量及乳铁蛋白表达无影响。

英文摘要:

Objective To evaluate the effect of Jieze No.1 Gel (JZ1) on the inner environment of vagina through observing its influences on vaginal levels of lactobacillus,glycogen and lactoferrin in mice. Methods One hundred female Kunming mice were randomized into 5 groups,the blank control (BC) group,the blank gel (BG) group,and the three JZ1 treated groups. They were treated via vagina with saline,matrix gel,high (2 mg/μL),middle(1 mg/μL) and low (0.5 mg/μL) dose JZ1 respectively in volume of 20 μL,72 h after being subcutaneously injected with physiological estradiol benzoate (E2) 2 μg. The 24 h and 72 h living lactobacillus in vaginal lavage fluid cultures were counted,the content of glycogen and the expression of lactoferrin in vaginal tissue were measured. Results No significant difference was found between the BC group and the three JZ1 treated groups in terms of lactobacillus-CFU,glycogen content and lactoferrin expression,and these indices detected at 24 h were not different to those detected at 72 h in the groups treated with various doses of JZ1 respectively (P>0.05). Conclusion Once application of JZ1 shows no effect on levels of lactobacillus,glycogen,and lactoferrin expression in the vagina of mice.

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