| 胡素敏,周鹏,傅骞,杨佳佳,姜山,何明,高学敏.五加补骨方对模拟失重大鼠消化道外源钙吸收的影响[J].中国中西医结合杂志,2009,(8):729-732 |
| 五加补骨方对模拟失重大鼠消化道外源钙吸收的影响 |
| Effect of Wujia Bugu Recipe on Intestinal Calcium ABSorption in Rats under Simulated Weightlessness Manner |
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| DOI： |
| 中文关键词: 41Ca 五加补骨方 模拟失重 钙吸收 |
| 英文关键词:41Ca Wujia Bugu Recipe simulated weightlessness ABSorption of calcium |
| 基金项目:国家自然科学基金资助项目(No.30500663);2006年教育部优秀人才支持计划(No.NCET-06-0124);2006年北京市科技新星(A)类计划(No.2006A49);国家基础科学人才培养基金(No.20070110) |
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| 中文摘要: |
| 目的研究五加补骨方对模拟失重大鼠消化道外源钙吸收的干预作用。方法雄性Wistar大鼠21只,随机分为3组,空白组、模型组、中药组,尾吊模拟失重,中药组以五加补骨方(含熟地黄、怀牛膝、黄芪、当归、牡蛎醋酸水解物等)水煎剂灌胃;适应期1周后进入模拟失重期,于模拟失重第11天一次性灌胃一定量41Ca示踪剂,连续收集72h粪便,加速器质谱法(acceleratormass spectrometry,AMS)分析粪便41Ca/40Ca值,电感耦合等离子体光谱法(inductively coupled plasma-atomic emission spectroscopy,ICP-AES)分析粪钙总量,计算41Ca粪排量、41Ca吸收量、肠钙吸收率。结果与空白组比较,模型组粪便41Ca/40Ca值及41Ca粪排量显著增高,41Ca吸收量及肠钙吸收率显著降低,差异有统计学意义(P<0.01);中药组粪便41Ca/40Ca值亦增高,但较模型组增高程度小,两组比较,差异有统计学意义(P<0.05)。与空白组比较,中药组各项指标差异均无统计学意义(P>0.05)。结论尾吊模拟失重可造成大鼠肠钙吸收显著下降,五加补骨方可不同程度改善模型大鼠肠钙吸收状况。 |
| 英文摘要: |
| Objective To investigate the intervention effect of Wujia Bugu Recipe (WBR,consisting of Radix Rehmanniae Praeparata,Radix Achyranthis bidentatae,Radix Astragali,Radix Angelicae sinensis,acetolytic substance of Concha Ostreae) on the intestinal ABSorption of exogenous calcium in rats under simulated Weightlessness manner. Methods Twenty-one male Wistar rats were randomly divided into 3 groups,the blank group,the model group,and the tested group treated with WBR via gastric perfusion. Simulated weightlessness manner was formed by tail suspending after rats′ entry into an one-week adaptation stage. And a definite dose of 41Ca tracer was given to each rat by gastric administration on the 11th day of the weightless stage. Their 72 h feces were collected for measuring ratio of 41Ca/40Ca by accelerator mass spectrometry (AMS),the total calcium in feces was determined by inductively coupled plasma-atomic emission spectroscopy (ICP-AES),the 41Ca feces content (FC) and intestinal ABSorption amount (AA),and then the calcium intestinal ABSorption ratio(AR)were analyzed and calculated. Results 41Ca/40Ca ratio and 41Ca FC were higher,41Ca AA and AR were lower in the model group than those in the blank group,the differences between groups were significant (P<0.01);while in the tested group,the differences of all above-mentioned indices with those in the blank group were insignificant,although the 41Ca/40Ca ratio showed somewhat increased,yet the increment was less than that in the model group (P<0.05). Conclusion Simulated weightlessness could lead to decrease of intestinal calcium ABSorption,which could be improved to some extent by Chinese herbal medicine. |
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