不同浓度凉血化瘀中药对脉络膜新生血管形态学的影响

目的评价不同浓度凉血化瘀中药对氪激光诱导棕色挪威(BN)大鼠脉络膜新生血管(CNV)形态的影响。方法48只BN大鼠一眼行氪激光(659nm)眼底光凝,激光功率、光凝斑直径和曝光时间分别为360mW、50μm及0.05s。随机分成空白对照组(生理盐水),中药高浓度[5.0g/(kg.d)]、中浓度[2.5g/(kg.d)]和低浓度[1.25g/(kg.d)]4组,每组12只,每天2次灌胃给药,连续21天。4组分别在光凝后第7、14、21天随机选取4只大鼠行眼底照像、荧光素眼底血管造影(FFA)检查;眼球标本行组织病理切片光镜、免疫组织化学染色观察。结果FFA显示:光凝后7～21天,空白对照组CNV荧光素渗漏面积(像素,-x±s)逐渐增大,而中药组荧光素渗漏面积无明显增加;第21天时,中药中浓度(52343.13±12973.92)、低浓度(66252.78±20659.71)组CNV荧光素渗漏面积明显低于空白对照组(91457.19±29309.11)和中药高浓度组(95973.40±53950.43),差异均有统计学意义(P<0.05),以中药中浓度组CNV荧光素渗漏面积最小。CNV厚度(μm,x-±s):空白对照组7～21天逐渐增厚,21天时达到高峰(55.3383±8.5036);而中药组14天时CNV厚度达到高峰,14+～21天CNV厚度变薄,21天时中药低浓度(38.9372±5.1728)、中浓度(38.8473±7.9862)、高浓度(40.0913±13.3448)组均变薄,与空白对照组同期(55.3383±8.5036)比较,差异均有统计学意义(P<0.05);以中药中浓度组最薄。结论凉血化瘀中药中浓度可有效抑制氪激光诱导BN大鼠的CNV增生,防止CNV荧光素渗漏。

英文摘要:

Objective To evaluate the influence of Chinese drugs for cooling blood and dissolving stasis(CBDS)in different concentrations on morphology of krypton laser induced choroidal neovascularization(CNV)in brown Norway(BN)rats.Methods Forty-eight rats received laser irradiation(659 nm)on fundus of one eye(power 360 mW,spot diameter 50 μm,time 0.05 s).They were divided into four groups equally:the control group(A)treated with normal saline,and the three CBDS groups treated respectively with high(B,5.0 g/kg),median(C,2.5 g/kg)and low(D,1.25 g/kg)dosage of CBDS,twice every day via gastric perfusion for 21 successive days.Fundus fluorescein angiography(FFA)on 4 selected rats in each group was performed at the 7th,14th and 21st day after photocoagulation,and histopathologic examination using light microscope with immuno-histochemical stain was conducted on them as well.Results FFA showed that CNV was firstly appeared on day 7 after photocoagulation,in Group A,it expanded gradually and reached the peak on day 21,but showed no significant expansion in the three CBDS groups.The fluorescein leakage in Group C(52 343.13±12 973.92 dots)and D(66 252.78±20 659.71 dots)was significantly less than that in Group A(91 457.19±29 309.11 dots)and B(95 973.40±53 950.43 dots)on day 21,all showing statistical significance(P<0.05).The variation of CNV in thickness showed that in Group A it increased gradually from day 7 and reached the peak on day 21(55.3383±8.5036 μm);but in the CBDS groups,the peak was reached on day 14,then became thinned gradually,on day 21,it was 40.0913±13.3448 μm in Group B,38.8473±7.9862 μm in Group C and 38.9372±5.1728 μm in Group D,all thinner than that in Group A significantly(P<0.05).Conclusion CDBS can effectively suppress the krypton laser induced CNV proliferation and prevent the CNV leakage in BN rats.

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