清胰Ⅱ号颗粒剂对急性坏死性胰腺炎大鼠肠道细菌移位的影响

作者	单位
肖青川	遵义医学院附属医院肝胆胰外科
兑丹华	遵义医学院附属医院肝胆胰外科
兰天罡	遵义医学院附属医院肝胆胰外科
李开伦	遵义医学院附属医院临床医学实验室

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中文摘要:

目的观察清胰Ⅱ号颗粒剂对急性坏死性胰腺炎(acute necrotizing pancreatitis,ANP)大鼠肠道细菌移位的影响并探讨其机理。方法将18只SD大鼠随机等分为假手术组、ANP组及治疗组,每组6只;ANP组及治疗组以30g/L牛磺胆酸钠逆行注入胰胆管制作大鼠ANP模型,造模术后1h,治疗组以250g/L清胰Ⅱ号颗粒剂灌胃给药(10mL/kg),6h1次,共3次,假手术组及ANP组用同等剂量生理盐水以相同方式灌胃。造模后24h,取大鼠肝脏、胰腺、脾脏、肠系膜淋巴结组织及回肠内容物行细菌培养及菌种鉴定,用real-time PCR检测回肠组织中高迁徙率族蛋白-1(high mobility group box1,Hmgb1)mRNA表达,ELISA测定回肠组织中一氧化氮(nitric oxide,NO)、内皮素-1(endothelin-1,ET-1)浓度,同时观察胰腺、回肠组织病理改变,测定回肠组织湿/干重比值。结果ANP组胰腺、回肠组织损伤明显,回肠组织Hmgb1mRNA的表达较假手术组上调(P<0.01),NO、ET-1浓度较假手术组升高(P<0.01),分别为(1.67±0.21)μmol/L、(102.18±9.19)ng/L,细菌移位至肝脏、胰腺、脾脏及肠系膜淋巴结;治疗组回肠组织中Hmgb1 mRNA的表达较ANP组下调(P<0.01),NO、ET-1浓度较ANP组降低(P<0.05),分别为(1.39±0.23)μmol/L、(83.15±5.39)ng/L,回肠病理损害程度减轻,细菌移位减少。结论Hmgb1、NO及ET-1浓度在ANP模型大鼠肠道细菌移位中可能具有重要作用,清胰Ⅱ号颗粒剂可能通过下调回肠组织中Hmgb1 mRNA的表达,降低NO、ET-1浓度,减轻胰腺、回肠组织的病理改变,从而抑制肠道细菌移位。

英文摘要:

Objective To observe the effects and mechanism of Qingyi Ⅱ Granules(QYG)on the bacterial translocation in rats with acute necrotizing pancreatitis(ANP).Methods Eighteen Sprague-Dawley rats were randomized equally into 3 groups,the sham-operated group(A),the ANP model group(B)and the treated group(C).Rats in Group B and C were established into ANP model by retrograde injection of 30 g/L sodium taucrocholate into pancreatobiliary duct.QYG was administered,beginning from 1 h after modeling,for three times(every 6 h)per day via intragastric infusion to Group C in dose of 10 mL/kg(250 g/L),while to the other two groups,equal volume of saline was infused instead.All animals were sacrificed 24 h after modeling.The contents in mesenteric lymph nodes and distant sites(liver,spleen,pancreas)were taken for bacterial culture and strain identification,the expression of high mobility group box 1(Hmgb1)mRNA in ileal tissue was assayed by real-time PCR;the levels of nitric oxide(NO)and endothelin-1(ET-1)were determined by ELISA;the wet/dry ratio of ileum was measured;and the pathologic features of pancreas and ileum were examined respectively.Results In Group B,evident pathological injury in pancreas and ileum was shown,expression of Hmgb1 mRNA up-regulated,levels of NO and ET-1 in ileum tissues increased to 1.67±0.21 μmol/L and 102.18±9.19 ng/L respectively,and the bacterial counts in the mesenteric lymph nodes and distant sites increased significantly.Compared with Group B,the level of NO and ET-1 reduced to 1.39±0.23 μmol/L and 83.15±5.39 ng/L,respectively in Group C,with all the above-mentioned abnormal changes alleviated significantly.Conclusion Levels of Hmgb1,NO and ET-1 might play important roles in the ANP model rats with intestinal bacterial translocation.QYG shows effects on preventing the intestinal bacterial translocation by way of down-regulate the Hmgb1 mRNA expression,lowering the concentration of NO and ET,and ameliorating the injury of pancreatic and ileum tissues.

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