祛瘀解毒颗粒治疗子宫内膜异位症患者卵泡液对小鼠胚胎发育的影响

作者	单位
李新玲	山东中医药大学附属医院中西医结合生殖与遗传中心
连方	山西省中医院妇科
刘延荷	山西省中医院妇科

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中文摘要:

目的观察经祛瘀解毒颗粒治疗后子宫内膜异位症(endom etriosis,EM)患者卵泡液对小鼠胚胎发育的影响。方法在常规培养液中分别加入治疗组EM患者卵泡液(祛瘀解毒颗粒)、对照组EM患者卵泡液及空白组(因输卵管因素行体外受精-胚胎移植)患者卵泡液,20只小鼠超排卵共收集189枚2-细胞鼠胚分别放入各组培养液进行培养,其中治疗组培养液70枚,对照组培养液60枚,空白组培养液59枚,观察和检测2-细胞鼠胚在体外发育至8细胞期、桑葚胚期和囊胚期的比率,及早期鼠胚优质胚胎数目和优质胚胎率。结果治疗组培养液有53枚鼠胚(75·71%,53/70)发育到8细胞期,48枚(68·57%,48/70)发育到桑葚胚期,45枚(64·28%,45/70)发育到囊胚期,与对照组培养液(56·67%,34/60、48·33%,29/60、35·00%,21/60)比较差异有统计学意义(P<0·05)。治疗组有61枚优质胚胎(87·14%),对照组为44枚(73·33%),两组比较差异有统计学意义(P<0·05)。结论EM患者卵泡液对早期鼠胚存在毒性作用,经祛瘀解毒颗粒治疗后EM患者卵泡液可提高早期鼠胚培养质量。

英文摘要:

Objective To observe the effect of human follicular fluid (HFF) from endometriosis (EM) patients treated with Quyu Jiedu Granule (QJG) on mouse embryonic development.Methods Cultured 2-cell mouse embryos were divided into three groups.To the medium of Group A (70 embryos),HFF from endometriosis patients treated with QJG was added;to that of Group B (60 embryos),HFF from endometriosis patients untreated with QJG,and to Group C (59 embryos),HFF from patients with fallopian tube obstruction was added.The percentages of embryos developed to 8-cell stage,morula stage and blastula stage were counted,and the early stage number and rate of high-quality embryo were measured.Results Among the 70 embryos in Group A,53 (75.71%) developed to 8-cell stage,48 (68.57%) to morula stage and 45 (64.28%) to blastula stage;while in the 60 embryos of Group B,the corresponding number (percentage) were 34 (56.67%),29 (48.33%),21 (35.00%),respectively,showing significant differences between groups (P<0.05).High-quality of embryo was 61 (87.14%) in Group A and 44 (73.3%) in Group B,the difference between groups also showed statistical significance (P<0.05).Conclusion HFF from endometriosis patients is toxic to 2-cell mouse embryos,but after treated by QJG,it could elevate the quality of cultured mouse embryo in the early stage.

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