| 李勇,张达利,王鹏,王健,凌昌全.人参皂苷对人肝细胞HL-7702内性激素受体水平的影响[J].中国中西医结合杂志,2009,(12):1110-1113 |
| 人参皂苷对人肝细胞HL-7702内性激素受体水平的影响 |
| Effect of Ginsenoside on Level of Sex Hormone Receptors in Human Liver Cell Line HL-7702 |
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| DOI： |
| 中文关键词: 人参皂苷 性激素受体 HL-7702细胞 |
| 英文关键词:ginsenoside sex hormone receptors HL-7702 cell |
| 基金项目:国家自然科学基金资助项目(No.30472271);上海市卫生局科技发展基金课题资助项目(No.054030);上海市教委基金资助项目(No.06cz2013); |
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| 中文摘要: |
| 目的观察人参皂苷(ginsenoside,GSS)对人肝细胞HL-7702内性激素受体水平的调节作用。方法以四唑盐比色法(MTT)观察细胞生长以确定药物浓度,应用免疫组化法,免疫蛋白印迹法(Westernblot)和RT-PCR法分析人参皂苷对HL-7702细胞性激素受体蛋白和其mRNA水平的影响。结果随GSS药物浓度增大,细胞内雌激素受体(estrogen receptor,ER)、孕激素受体(progesterone receptor,PR)、雄激素受体(androgen receptor,AR)蛋白及mRNA表达水平均逐渐增高,与溶剂对照组比较,差异有统计学意义(P<0.05),且细胞内性激素受体蛋白及mRNA表达呈剂量依赖性增加。结论GSS可上调HL-7702细胞内性激素受体蛋白及mRNA的表达。 |
| 英文摘要: |
| Objective To investigate the effect of ginsenoside(GSS) in regulating level of sex hormone receptors in human liver cell line HL-7702.Methods The growth of HL-7702 were detected by MTT [3-(4,5-Dimethylthiaz ol-2-yl)-2,5-diphenyltetrazolium bromide] assay for choosing the available concentration of GSS;and the effect of GSS on sex hormone receptors in HL-7702 cells were detected by immuno-histochemistry,Western blot and RT-PCR.Results GSS significantly enhance the protein and mRNA expressions of estrogen receptor(ER),progesterone receptor(PR),androgen receptor(AR) in HL7702 cell in a dose-dependent manner,the levels of expressions in the GSS treated group were higher than those in the solvent control group respectively(P<0.05).Conclusion GSS can up-regulate the protein and mRNA expressions of sex hormone receptors in HL-7702. |
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