| 曹炜,姜泉,吴振宇,张云,吴广均,莫捷,宋爱武.风湿清对Ⅱ型胶原诱发类风湿关节炎大鼠白细胞介素-4、γ-干扰素及趋化因子的影响[J].中国中西医结合杂志,2009,(12):1114-1116 |
| 风湿清对Ⅱ型胶原诱发类风湿关节炎大鼠白细胞介素-4、γ-干扰素及趋化因子的影响 |
| Effect of Fengshiqing on Interleukin-4,γ-Interferon and Chemotactic Factor in Rats with Collagen TypeⅡ Induced Rheumatoid Arthritis |
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| DOI： |
| 中文关键词: 风湿清 类风湿关节炎 白细胞介素-4 γ-干扰素 趋化因子 |
| 英文关键词:Fengshiqing rheumatoid arthritis interleukin-4 γ-interferon chemotactic factor |
| 基金项目:国家自然科学基金资助项目(No.30873250) |
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| 中文摘要: |
| 目的了解风湿清对类风湿关节炎大鼠血清及滑膜细胞培养上清液中白细胞介素4(IL-4)、γ干扰素(γ-IFN)、巨噬细胞趋化因子1α(MIP-1α)、Ⅱ型胶原抗体(CⅡ抗体)的调节作用,探讨清热活血法治疗类风湿关节炎的作用机理。方法CⅡ抗体加弗氏完全佐剂诱发类风湿关节炎大鼠动物模型,ELISA方法检测大鼠血清及滑膜细胞培养上清液中CⅡ抗体、IL-4、γ-IFN及MIP-1α的含量。结果与正常组比较,模型组血清CⅡ抗体含量均明显升高(P<0.01),模型14天、28天组血清和滑膜细胞培养上清液中IL-4含量下降,γ-IFN、MIP-1α含量升高,差异有统计学意义(P<0.05或P<0.01);与模型组同期比较,风湿清14天组血清和滑膜细胞培养上清液IL-4含量升高、滑膜细胞培养上清液MIP-1α含量下降(P<0.05或P<0.01);风湿清28天组血清和滑膜细胞培养上清液MIP-1α含量均下降(P<0.05)。结论风湿清能明显上调类风湿关节炎大鼠血清及滑膜局部IL-4含量,对血清及滑膜局部γ-IFN有下调趋势,能明显下调全身和局部滑膜细胞MIP-1α的高表达,从而维持Th1/Th2细胞平衡,抑制滑膜局部的细胞及体液免疫反应,减轻慢性关节滑膜炎及血管炎病变。 |
| 英文摘要: |
| Objective To study the regulatory effect of Fengshiqing(FSQ) on interleukin 4(IL-4),γ-interferon(γ-IFN),macrophage inflammatory protein 1α(MIP-1α) and collagen type Ⅱ antibody(CⅡAb) in serum and supernatant of synovial cell culture of rat with rheumatoid arthritis(RA),to explore the mechanism of action of clearing-heat and activating blood method for treatment of RA.Methods RA rat model was induced by CⅡ Ab combined with Freund′s complete adjuvant,and the levels of IL-4,γ-IFN,MIP-1α and CⅡAb in serum and supernatant of synovial cell were detected by ELISA.Results As compared with the normal group,serum level of CⅡAb in the model group was significantly higher(P<0.01),serum and supernatant contents of IL-4 on the 14th and 28th day of modeling were lower and those of γ-IFN and MIP-1α were higher,the difference showed statistical significance(P<0.05 or P<0.01).After being treated with FSQ and IL-4 contents in serum and supernatant as well as MIP-1α in supernatant restored on the 14th day(P<0.05 or P<0.01),while all the indexes restored on the 28th day.Conclusion FSQ could evidently up-regulate the level of IL-4 and down-regulate that of MIP-1α in serum and local synovial membrane in RA rats,and shows a suppressive trend of γ-IFN,so as to maintain the Th1/Th2 equilibrium,suppress the cellular and humoral immune response in the local synovial membrane,and alleviate the chronic changes of arthritis,synovitis and vasculitis. |
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