| 高冬,陈文元,吴立娅,郑良朴,林薇,宋军.血府逐瘀汤诱导内皮细胞促血管新生的基因调控研究[J].中国中西医结合杂志,2010,30(2):153-156 |
| 血府逐瘀汤诱导内皮细胞促血管新生的基因调控研究 |
| Effect of Xuefu Zhuyu Decoction in Inducing Angiogenesis Gene Regulation of Endothelial Cell Line ECV304 |
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| DOI： |
| 中文关键词: 血府逐瘀汤 血管新生 基因芯片 |
| 英文关键词:Xuefu Zhuyu Decoction angiogenesis gene chip microarray |
| 基金项目:福建省自然科学基金资助(No.2007J0101);福建省“中西医结合基础与临床”创新团队项目(No.CKJ200801);福建省科技厅项目(No.2005K052) |
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| 中文摘要: |
| 目的研究血府逐瘀汤促进内皮细胞参与血管新生的机制。方法通过血府逐瘀汤含药血清对内皮细胞ECV304增殖、细胞周期、迁移和体外成血管的影响,明确药物具有显著的促血管新生作用,并运用基因芯片技术分析药物对血管新生各调控因子的作用。结果2.5%血府逐瘀汤含药血清作用48h能显著促进ECV304细胞活性、增加S期细胞数目,诱导内皮细胞迁移和促体外成血管作用。在此药物作用条件下,血府逐瘀汤上调4个,下调10个血管新生调控基因的表达。结论血府逐瘀汤促ECV304参与血管新生的机制复杂,呈多途径、多靶点现象。 |
| 英文摘要: |
| Objective To study the acting mechanism of endothelial cell line ECV304 in regulating angiogenesis induced by Xuefu Zhuyu Decoction (XFZY). Methods The angiogenesis effect of XFZY-contained serum (XFZY-CS) was confirmed by observing its impact on proliferation, cell cycle, migration of ECV304 and on vascular neogenesis in vitro. Then the effect of XFZY on various angiogenesis controlling factors was analyzed with gene chip microarray technique. Results Treatment of XFZY-CS in 2.5% concentration for 48 h showed evident actions of enhancing ECV304 activity, increasing cell numbers of S phase, inducing cell migration and promoting the in vitro angiogenesis. Meanwhile, expressions of four angiogenesis controlling genes were up-regulated and 10 were down-regulated. Conclusion The angiogenesis mechanism of ECV304 induced by XFZY is complex, it shows a multi-pathway and multi-target feature. |
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