梗阻性黄疸大鼠血栓素A<sub>2</sub>与前列环素I<sub>2</sub>变化及丹参的干预作用

作者	单位
秦琦瑜	河北医科大学第一医院肝胆外科
陈进军	河北医科大学第一医院肝胆外科
张庆富	河北医科大学第一医院烧伤整形科
李勇	河北医科大学第四医院外三科
王车江	河北医科大学第一医院烧伤整形科

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中文摘要:

目的观测梗阻性黄疸(OJ)大鼠血浆血栓素A2(TXA2)、前列环素I2(PGI2)及肾皮质微循环的变化,探讨OJ肾损伤的机制及丹参的治疗作用。方法将SD大鼠分为假手术组、胆总管结扎组(模型组)及丹参干预组(丹参组),每组分3、7、10天3个时相,每个时相8只。测定血浆TXA2、PGI2、血尿素氮(BUN)及肌酐(Cr)水平,计算T/P比值。应用WX-9型微循环显微镜观测各组相应时相肾皮质微血管管径的变化。结果模型组与假手术组比较各时相T/P值升高,肾皮质微血管收缩,7、10天血BUN、Cr水平升高(P<0.05),且随胆道梗阻时间延长变化更加明显(P<0.05)。丹参组与模型组比较各时相T/P值降低,7、10天肾皮质微血管扩张,10天血BUN及Cr水平下降(P<0.05)。结论血T/P比值升高及肾脏微循环障碍是OJ肾损伤的重要原因,丹参注射液对梗阻性黄疸肾损伤有干预作用。

英文摘要:

ObjectiveTo observe the alteration of plasmal levels of thromboxane A2(TXA2) and prostacyclin(PGI2) as well as changes of microcirculation in renal cortex of obstructive jaundice model rats,and to study the effect of Radix Salviae miltiorrhizae(SM) on them.MethodsSD rats were randomly divided into three groups: the sham operation group(A),the common bile duct ligation model group(B),and the SM treated model group(C).Their blood plasma TXA2/PGI2 ratio(T/P),blood levels of urea nitrogen(BUN) and creatinine(Cr) were determined respectively in batches(8 rats from each group) on the 3rd,7th and 10th day,their capillary caliber(CC) in renal cortex was measured at the same time points using WX-9 type microcirculation microscope.ResultsCompared with Group A,T/P was higher and CC was smaller in Group B at all the time points.Levels of BUN and Cr increased on day 7 and day 10 after modeling(P<0.05),and they were increasing markedly along with the longation of the obstructive time(P<0.05).As compared with Group B,T/P was lower and CC was expanded in Group C,with levels of BUN and Cr lowered on day 10(P<0.05).ConclusionT/P elevation and renal microcirculation obstacle are the important factors for inducing renal injury in obstructive jaundice,and SM shows a protective effect on kidney against the injury.

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