大鼠颅脑损伤后心肌损害和线粒体锰超氧化物歧化酶活性改变及银杏叶提取物的干预作用

Myocardial Damage and Change of Mitochondrial Mn-Superoxide Dismutase Activity in Craniocerebral Injured Rats and the Interventing Effect of Ginkgo Biloba Extract

免费下载全文查看/发表评论下载PDF阅读器

DOI：

中文关键词: 颅脑损伤心肌损害锰超氧化物歧化酶银杏叶提取物

英文关键词:craniocerebral injury myocardial damage Mn-superoxide dismutase Ginkgo biloba extract

基金项目:国家自然科学基金资助项目(No.30600637)

作者	单位
郝春艳	山西医科大学第一医院老年病科
王凤芝	山西医科大学第二医院内科
段虎斌	山西医科大学第一医院老年病科

摘要点击次数: 1349

全文下载次数: 820

中文摘要:

目的研究大鼠颅脑损伤后心肌损害和心肌线粒体锰超氧化物歧化酶(Mn-SOD)活性的变化及银杏叶提取物(GBE)对其影响。方法采用液压冲击建立颅脑损伤模型和药物干预模型,24 h动态监测心电图变化,测定血清肌酸磷酸激酶同工酶(CK-MB)的含量和心肌线粒体Mn-SOD的活性改变,并观察心肌HE染色的病理形态学改变。结果大鼠颅脑损伤后心电图异常发生率显著增高,血清CK-MB含量升高(P<0.05),心肌细胞线粒体Mn-SOD活性降低(P<0.05),且心肌细胞线粒体Mn-SOD活性与血清CK-MB含量呈负相关(r=-0.997,P<0.05)。GBE预处理后可以使大鼠颅脑损伤后心电图异常发生率明显降低(P<0.01),血清CK-MB含量降低(P<0.05),心肌细胞线粒体Mn-SOD活性升高(P<0.05),心肌的病理损伤程度减轻。结论大鼠颅脑损伤可引起明显的心肌损害,颅脑损伤后心肌损害的发生可能与心肌细胞线粒体抗氧化应激水平降低有关,GBE对大鼠颅脑损伤后心肌损害有保护作用。

英文摘要:

ObjectiveTo investigate the myocardial damage and changes of myocardial mitochondrial Mn-superoxide dismutase(Mn-SOD) activity in craniocerebral injured rats and the effect of Ginkgo biloba extract(GBE) on them.MethodsCraniocerebral injured rats model was established by fluid-percussion and treated with GBE.The dynamical changes of electrocardiograph(ECG) in 24 h were monitored,the serum level of MB isoenzyme of creatine kinase(CK-MB) and the change of myocardial mitochondrial Mn-SOD activity as well as the pathologic changes of myocardium(HE staining) were observed.ResultsThe occurrence of ECG abnormality obviously increased in the injured rats,accompanied with increased serum CK-MB(P<0.05) and decreased myocardial Mn-SOD levels(P<0.05),and the Mn-SOD activity was negatively correlated with the level of CK-MB(r=-0.997,P<0.05).Pretreatment of GBE resulted in the decrease of ECG abnormality occurrence(P<0.01),serum CK-MB level(P<0.05),and degree of myocardial damage,as well as the increase of Mn-SOD activity in post-craniocerebral injured rats.ConclusionsCraniocerebral injury can result in distinct myocardial damage,which is possibly correlated with the lowering of anti-oxidation stress level of myocardial cellular mitochondria.GBE possesses the protective effect on myocardial damage after craniocerebral injury.

关闭