杜兰芳,杜永平,徐晖,王文挺,胡三觉.延胡索乙素对大鼠慢性压迫背根节神经元自发放电的影响[J].中国中西医结合杂志,2010,30(4):388-392 |
延胡索乙素对大鼠慢性压迫背根节神经元自发放电的影响 |
Effect of 1-Tetrahydropalmatine on Spontaneous Electric Discharge Induced by Chronic Dorsal Root Ganglion Neurons Compression in Rats |
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DOI: |
中文关键词: 延胡索乙素 自发放电 慢性压迫 背根节 |
英文关键词:1-tetrahydropalmatine spontaneous electric discharge chronic compression dorsal root ganglion |
基金项目:国家自然科学基金重点资助项目(No.3053026);国家自然科学基金资助项目(No.30870830) |
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中文摘要: |
目的观察延胡索乙素(l-THP)对大鼠慢性压迫背根节神经元A类纤维自发放电的影响。方法采用单纤维记录大鼠慢性压迫背根节神经元自发放电的方法,观察l-THP对自发放电的影响,以及浓度-效应关系。结果实验中记录到单纤维A类神经元自发放电84根。在A类神经元自发放电中有25根自发放电为周期节律,59根为非周期节律。l-THP(100μmol/L)分别引起16.0%(4/25)的周期节律放电神经元和67.8%(40/59)非周期节律放电神经元放电减少(P<0.01,l-THP干预前后配对t检验)。l-THP对受损背根节(DRG)神经元自发放电的抑制作用具有剂量依赖关系,随着浓度的增大抑制神经元自发放电的时间越快,自发放电恢复所需的时间也越长。其中有57.1%(24/42)的DRG神经元自发放电在洗脱后20 min内可以恢复,42.8%(18/42)的DRG神经元自发放电在洗脱后经过3 h始终没有恢复。结论l-THP对大鼠慢性压迫背根节神经元A类纤维的自发放电有抑制作用。 |
英文摘要: |
Objective To study the effect of 1-tetrahydropalmatine(1-THP) on the spontaneous electric discharge(SED) induced by chronic dorsal root ganglion neurons compression.Methods Using single fiber recording method,the SED of 84 neurons class A induced by compression were recorded.The effect of 1-THP on the SEDs and its relation with concentration were observed.Results In the 84 SED of neurons,25 showed periodical rhythmicity(PR) and 59 showed non-periodic rhythmicity(non-PR).1-THP(100 μmol/L) inhibited SED in 16.0%(4/25) of neurons with PR and 67.8%(40/59) of neurons with non-PR(P<0.01) in an effect-dose dependent manner,the higher the concentration of 1-THP,the more the inhibition,with quicker inhibiting initiation and longer time needed for recovery.SED in 57.1% neurons were recovered 20 min after elution,but unrecovered even after 3 h in the others.Conclusion 1-THP shows inhibitory effect on the A-fiber SED induced by chronic dorsal root ganglion neurons compression. |
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