黄芪注射液对新生儿窒息后血清诱导人肾小管细胞损伤信号机制的影响

作者	单位
熊涛	泸州医学院附属医院新生儿科
董文斌	泸州医学院附属医院新生儿科
王明勇	泸州医学院附属医院传染与免疫研究室
邓存良	泸州医学院附属医院传染与免疫研究室

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中文摘要:

目的从细胞蛋白水平和分子水平研究黄芪对新生儿窒息后血清诱导人近曲肾小管上皮细胞(HK-2细胞株)损伤的信号传导机制的影响〔是否通过激活核因子κB(NF-κB)信号途径〕。方法以HK-2细胞株为研究对象,以20%窒息血清作为攻击因素,攻击前分为空白对照组和黄芪预处理组,在攻击后15min,1、2 h分为相应时间的血清攻击组和黄芪预处理组后攻击组。通过激光共聚焦显微镜观察间接免疫荧光染色后NF-κB核转位以及免疫印迹(Western blot)观测NF-κB抑制亚基I-κBα量的变化。结果攻击前与空白对照组比较,黄芪预处理组NF-κB核转位和I-κBα量无改变;而攻击后各组与空白组对照组比较,NF-κB核转位显著增加和I-κBα量显著减少,以攻击后1 h最明显;各时点黄芪预处理组后攻击组与空白攻击组比较,NF-κB核转位显著减少和I-κBα量显著增加。结论黄芪预处理能够抑制窒息后血清所致后续NF-κB激活。

英文摘要:

Objective To investigate,from cytoprotein and molecular levels,the action mechanism of astragalus Injection(ASI) on the signal conduction of human renal tubular cells(HK-2) injury induced by neonatal postasphyxial-serum(NPS),whether it is through activating the nuclear factor κB(NF-κB) signaling pathway.Methods Taking HK-2 as the target cell and the 20% NPS as the attacking factor,the experiment was conducted by dividing the target cells into two groups before attacking,the blank control group and the ASI pretreated group.The nuclear translocation of NF-kB was detected by confocal microscopy with indirect immunofluorescence stain,and the amount of NF-κB inhibitor subunit(I-κBα) was detected by Western blot before attacking.The detections were repeated at various time points in the experiment,i.e.,15 min,1 h and 2 h after attacking,respectively.Results Before attacking,the nuclear translocation of NF-kB and the amount of I-κBα were not different in the two groups.But the former increased and the latter decreased significantly in the ASI group at all the time points after attacking with the topmost changes presented at 1 h after attacking,and significantly different to those in the control group at corresponding time points.Conclusion ASI pretreatment could inhibit the activation of NF-kB induced by postasphyxial-serum.

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