薯蓣皂苷元活性成分对心肌细胞损伤的保护作用及机制研究

作者	单位
宁宗	广西医科大学第一附属医院
李贻奎	中国中医科学院西苑医院
周燕	广西医科大学药理教研室
张荣利	北京大学分子医学研究室

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中文摘要:

目的观察薯蓣皂苷元(MPD)活性成分对缺氧再复氧引起的心肌细胞损伤的保护作用,并研究其作用机制。方法采用建立缺氧再复氧引起心肌细胞损伤的模型,将细胞分为正常组、对照组和MPD组,正常组不进行缺氧再复氧和药物干预,对照组进行缺氧再复氧无MPD干预,MPD组进行缺氧再复氧和MPD干预,观察乳酸脱氢酶(LDH)和心肌细胞膜ATP酶。另外对正常心肌细胞经MPD的处理后,检测钠钙交换器(NCX)的mRNA表达量。结果与对照组比较,加入MPD(10μg/mL、50μg/mL)处理后,细胞的损伤程度减轻。经缺氧再复氧后MPD组的钠钾ATP酶和钙镁ATP酶的活性显著高于对照组。与对照组比较,NCX的mRNA表达量经MPD处理后降低(P<0.05)。结论MPD可能通过维持心肌细胞膜钠泵和钙泵功能,共同影响心肌细胞钙离子跨膜转运,维持细胞内低钙离子的环境。

英文摘要:

Objective To study the effect and mechanism of methyl protodioscin(MPD),an active ingredients of yamogenin,in protecting cardiomyocytes(CMC) against anoxia/reoxygenation(A/R) injury.Methods Cultured CMCs of neonatal SD rats were randomly divided into three groups,cells in Group A were untreated normal cells,cells in Group B and C were made to injury CMC model by A/R,and only those in Group C were treated with MPD.Levels of ATPase activity and lactate dehydrogenase(LDH) in cell membrane of CMCs were determined.Besides,the mRNA expression of sodium-calcium exchanger(NCX) in MPD treated CMCs was detected.Results As compared with Group B,the degree of CMC injury was significantly milder and the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase were higher in Group C after cells were treated with MPD in concentration of 10 μg/mL and 50 μg/mL.The mRNA expression of NCX in CMCs was down-regulated after MPD treatment(P<0.05).Conclusion MPD could maintain the low calcium internal environment in CMCs by way of protecting the membranous function of Na+-pump and Ca2+-pump,and influencing the Ca2+ transmembrane transportation in CMCs.

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