淫羊藿苷对骨髓间充质干细胞骨向诱导的实验研究

作者	单位
吴昊	暨南大学附属第一医院骨科
查振刚	暨南大学附属第一医院骨科
姚平	暨南大学医学院
熊高鑫	暨南大学附属第一医院骨科
郇松玮	暨南大学附属第一医院骨科
束志勇	暨南大学附属第一医院骨科

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中文摘要:

目的通过研究淫羊藿苷(icariin,ICA)对骨髓间充质干细胞(bone mesenchymal stem cells,BM-SCs)增殖和骨向分化能力的影响,并观察其促BMSCs骨向分化过程中对细胞因子转化生长因子β1(TGF-β1)、骨形成蛋白-2(BMP-2)表达的影响,分析可能的作用途径,为阐释其防治骨质疏松(osteoporosis,OP)的作用机理提供实验依据,并为以TGF-β1、BMP-2作为筛选防治OP药物进行实验研究。方法以碱性磷酸酶(ALP)等指标确定干预药物不同浓度梯度中最佳促BMSCs骨向分化剂量;分组实验,通过检测诱导分化过程中ALP和钙化结节等指标的表达,来观察其对BMSCs骨向分化能力的影响;采用ELISA法检测骨向分化过程中TGF-β1、BMP-2的表达。结果(1)ICA最佳促BMSCs骨向分化浓度为1×10-9mol/L。ICA10-9组能促进成骨指标的表达和TGF-β1和BMP-2分泌增加;(2)能促BMSCs骨向分化能力的ICA各诱导组均伴随TGF-β1和BMP-2分泌增加。结论ICA能促进BMSCs骨向分化;在BMSCs骨向分化过程中,上调TGF-β、BMP-2的表达量可能是各干预药物促进BMSCs骨向分化的作用机制之一。

英文摘要:

Objective To research the effect of icariin(ICA) on the proliferation and differentiation of bone mesenchymal stem cells(BMSCs) and to study its influence on the expressions of transforming growth factor-β1(TGF-β1) and bone morphogenetic protein-2(BMP-2) in the progress of BMSCs differentiating into osteoblast,for providing an experimental evidence to explain the mechanism of ICA,also for exploring the feasibility of establishing a platform upon TGF-β1 and BMP-2 to screen out the medicine in preventing and treating osteoporosis.Methods After the most effective concentration of ICA for promoting the differentiation of BMSCs into osteoblast was judged with the indices like alkaline phosphatase(ALP),etc.,a grouped experiment was conducted for the sake of studying the effect and mechanism of ICA in its process of inducing BMSCs differentiation into osteoblast through detecting expression of ALP and calcium nodes,as well as the expressions of TGF-β1 and BMP-2 with ELISA.Results The most effective concentration of the ICA on the BMSCs differentiation was judged as 1×10-9mol/L,ICA of that concentration showed effects in enhancing the expressions of osteoblast-indices and increasing the secretion of TGF-β1 and BMP-2.Besides,the increase of TGF-β1 and BMP-2 was revealed in all the groups,in which ICA showed its influence visibly.Conclusion ICA could promote the differentiation of BMSCs into osteoblast;the up-regulation of TGF-β1 and BMP-2 expressions is possibly one of the action mechanisms of various interventional drugs in their differentiation promoting progress.

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