鹤蟾片诱导人肺腺癌A549细胞凋亡的实验研究

作者	单位
熊绍权	广州中医药大学第一附属医院肿瘤科
周岱翰	广州中医药大学第一附属医院肿瘤科
林丽珠	广州中医药大学第一附属医院肿瘤科

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中文摘要:

目的研究鹤蟾片对A549人肺腺癌细胞凋亡的影响。方法制备大鼠含药血清,MTT法检测鹤蟾片含药血清对A549细胞的生长抑制率。利用PI染色观察和流式细胞术研究该含药血清对A549细胞凋亡的影响。RT-PCR检测含药血清对细胞表皮生长因子受体(epidermal growth factor receptor,EGFR)mRNA表达的调节作用。结果鹤蟾片含药血清明显抑制A549细胞生长,并使该细胞呈凋亡形态学改变。10%、20%含药血清组的细胞凋亡率为20.5%、33.2%,与对照组(6.1%)比较,差异均有统计学意义(P<0.05)。含药血清组的细胞EGFR表达明显低于对照组(P<0.05)。结论鹤蟾片具有诱导人肺腺癌A549细胞凋亡的作用,分子机制可能与抑制EGFR基因的转录有关。

英文摘要:

Objective To study the apoptosis inducing effects of Hechanpian (HCP) on human lung adenocarcinoma A549 cells. Methods HCP containing rat serum was prepared and applied on A549 cells. The cell growth inhibition rate was tested by MTT assay; the effect of HCP on cell apoptosis was observed with Propidium iodide (PI) staining and flow cytometry analysis; the mRNA expression of epidermal growth factor receptor (EGFR) was detected through RT-PCR.Results The growth of A549 cells was obviously inhibited after being treated by HCP containing serum,and the cells presented an apoptotic change. The cell apoptosis rate after treated by serum containing 10% and 20% HCP was 20.5% and 33.2%,respectively,significantly higher than that in the control (6.1% in cells didn’t treated with HCP,P<0.05). Compared with control,EGFR mRNA expression in HCP treated cells was significantly lower (P<0.05).Conclusion HCP has apoptosis inducing effect on A549 cell,and its molecular mechanism is probably correlated with the inhibition of EGFR gene transcription.

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