麝香提取物对大鼠皮层神经细胞炎性损伤的保护作用

作者	单位
石进峰	郑州大学第一附属医院神经内科
张博爱	郑州大学第一附属医院神经内科
贾延劼	郑州大学第一附属医院神经内科
沈雷	郑州大学第一附属医院神经内科
关文娟	郑州大学第一附属医院神经内科
赵二义	郑州大学第一附属医院神经内科
文全庆	郑州大学第一附属医院神经内科

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中文摘要:

目的研究麝香提取物(musk extract,ME)对脂多糖(LPS)诱导大鼠大脑皮层神经细胞炎性损伤的保护作用及其可能机制。方法分别培养新生大鼠大脑皮层神经细胞和星形胶质细胞(AC),使用终浓度为10mg/LLPS及ME18mg/L、36mg/L、72mg/L、144mg/L的分别作用于纯化的星形胶质细胞24h,收集条件培养液(ACM)。在神经细胞培养体系中分别加入ACM,采用MTT法及AO/EB染色法测定神经细胞存活率和凋亡率;ELISA法检测ACM中所含炎症因子的变化。结果 ME18mg/L、36mg/L、72mg/L及144mg/L组的神经细胞存活率(%)分别为52.55±3.52、55.77±2.36、64.89±3.45及73.67±1.80,较模型组(43.62±4.51)均显著提高(P<0.05);细胞凋亡率(%)分别为68.11±2.16、44.27±3.68、32.56±2.14及21.89±2.46,与模型组(71.33±3.25)比较,均显著下降(P<0.05,P<0.01);ME组ACM中IL-6呈指数降低,且均呈剂量依赖性。结论麝香提取物对脂多糖所致神经细胞炎性损伤具有显著的保护作用,以144mg/L浓度最佳,其可能通过减少星形胶质细胞分泌IL-6起作用。

英文摘要:

Objective To investigate the protective effects of musk extract (ME) and its possible mechanism on rat’s cerebral cortical neurons with inflammatory injury induced by lipopolysaccharide (LPS). MethodsNeurons and astrocytes from newborn rat cerebral cortex were cultured in vitro respectively,and the astrocyte conditioned medium (ACM),obtained by treating astrocytes with 10 mg/L LPS and different concentrations of ME for 24 h,was added in the culture fluid of neurons. The survival rate and apoptotic rate of neurons were measured by MTT method and AO/EB stain; and the changes of inflammatory factors in the ACM were determined by ELISA. Results The survival rate (%) of neurons treated by ACM with ME in concentrations of 18 mg/L,36 mg/L,72 mg/L and 144 mg/L was 52.55±3.52,55.77±2.36,64.89±3.45 and 73.67±1.80,respectively,significantly higher than that in the model neurons (43.62±4.51,P<0.05),while the apoptotic rate (%) in them,68.11±2.16,44.27±3.68,32.56±2.14 and 21.89±2.46,respectively,was significantly lower than that in model neurons (71.33±3.25,P<0.05 or P<0.01). Level of IL-6 was decreasing along with the raising of ME concentration in the ACM,showing a concentration-dependent state. Conclusion ME shows apparent protective effect on neurons against inflammatory injury,especially in a high concentration (144 mg/L),which may be associated with the reduction of IL-6 secreted by astrocytes.

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