中药联合窄谱中波紫外线对寻常型银屑病表皮中B细胞淋巴瘤/白血病-2蛋白、天冬氨酸特异性半胱氨酸蛋白酶-3及生存素的影响

Effect of Combined Therapy with Chinese Drug and Narrow Broad Ultraviolet B on Bcl-2,Caspase-3 and Survivin in Skin Lesion with Psoriasis Vulgaris

免费下载全文查看/发表评论下载PDF阅读器

DOI：

中文关键词: 寻常型银屑病中药窄谱中波紫外线 B细胞淋巴瘤/白血病-2蛋白天冬氨酸特异性半胱氨酸蛋白酶-3 生存素

英文关键词:psoriasis vulgaris Chinese medicine narrow broad ultraviolet B Bcl-2 Caspase-3 survivin

基金项目:

作者	单位
秦兰英	河北省沧州市人民医院皮肤科
邢卫斌	河北省沧州市人民医院皮肤科
叶文静	河北省沧州市人民医院皮肤科
李玉平	河北医科大学第二医院皮肤科

摘要点击次数: 1330

全文下载次数: 671

中文摘要:

目的探讨中药联合窄谱中波紫外线(NB-UVB)在调节寻常型银屑病皮损中角质形成细胞凋亡的可能作用。方法选择30例寻常型银屑病患者给予中药银屑病方联合NB-UVB治疗,疗程8周,取治疗前后标本,用免疫组化方法检测正常人(20名)及患者(30例)治疗前后皮损中B细胞淋巴瘤/白血病-2(Bcl-2)蛋白、天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)、生存素(survivin)的表达。结果寻常型银屑病患者皮损中Bcl-2(7.50±2.01)的表达明显低于正常组(12.65±2.83),Caspase-3(21.73±3.70)的表达明显高于正常组织(8.55±2.16),survivin(23.90±2.82)的表达明显高于正常组织(7.06±1.96,P<0.01);治疗后Bcl-2(13.63±2.14)表达上调,Caspase-3(11.70±2.44)、survivin(12.46±1.80)表达下调(P<0.01),三者均有向正常组织表达转化的趋势。银屑病面积和严重程度指数(PASI)评分由治疗前(14.24±3.42)下降至治疗后(3.52±1.07,P<0.01))。结论中药银屑病方联合NB-UVB可能通过调节Bcl-2、Caspase-3、survivin的表达而调节角质形成细胞的凋亡达到治疗目的。

英文摘要:

Objective To reveal the possible role of combined therapy with Chinese drug and narrow broad ultraviolet B(NB-UVB)on keratinocytes apoptosis in skin lesion of psoriasis vulgaris(PV).Methods Skin samples were taken from 20 healthy subjects and 30 PV patients before and after they received the combined therapy for 8 weeks.SP immunohistochemical method was used to detect the expressions of Bcl-2, Caspase-3 and survivin in the samples.Results As compared with those in the normal skin,expression of Bcl- 2 in PV skin was significantly lower(7.50±2.01 vs.12.65±2.83),while expression of Caspase-3(21.73±3.70 vs.8.55±2.16),and survivin(23.90±2.82 vs.7.06±1.96)were higher(all P<0.01).After treatment, in skin of PV,Bcl-2 expression increased to 13.63±2.14,Caspase-3 and survivin decreased to 11.70±2.44 and 12.46±1.80,respectively(all P<0.01),showing a normalizing trend.Moreover,patients’ psoriasis area and severity index(PASI)score decreased from 14.24±3.42 before treatment to 3.52±1.07 after treatment (P<0.01).Conclusion The curing effect of the combined therapy with Chinese drug and NB-UVB in treating PV is possibly realized by way of regulating Bcl-2,Caspase-3 and survivin expressions to adjust keratinocyte apoptosis.

关闭