乳癌术后方对HER2/neu转基因小鼠乳腺癌发生中免疫应答的影响

作者	单位
吴雪卿	上海中医药大学附属曙光医院中医乳腺外科
万华	上海中医药大学附属曙光医院中医乳腺外科
李欣荣	上海市复旦大学附属妇产科医院
冯佳梅	上海中医药大学附属曙光医院中医乳腺外科
姜晓燕	上海中医药大学附属曙光医院中医乳腺外科
瞿文超	上海中医药大学附属曙光医院中医乳腺外科
高晴倩	上海中医药大学附属曙光医院中医乳腺外科

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中文摘要:

目的探讨癌基因人类表皮生长因子受体2(HER2/neu)在乳腺癌发生中引发的免疫应答和乳癌术后方在防治乳腺癌中的免疫学机制。方法选用HER2/neu转基因自发乳腺癌小鼠模型,自5周龄起灌饲中药乳癌术后方,观察小鼠的乳腺癌发生情况,并在15及25周龄时检测小鼠肿瘤发生过程中T细胞免疫反应及相关细胞因子的变化。结果乳癌术后方能够明显延缓和减少转基因小鼠原发乳腺癌的生成,同时还能上调荷瘤小鼠脾淋巴细胞中T细胞含量,促进荷瘤小鼠T淋巴细胞增殖作用,并可诱导白细胞介素-2(IL-2)、白细胞介素-12(IL-12)和干扰素-γ(IFN-γ)等细胞因子的分泌。结论HER2/neu转基因小鼠与正常同品系未转基因小鼠的免疫功能存在着明显的差别。乳癌术后方预防和延缓乳腺癌发生的作用可能是通过提高转基因小鼠的自身抗肿瘤免疫反应来实现的。

英文摘要:

Objective To explore the immune response induced by HER2/neu oncogene in the breast cancer(BC)carcinogenesis process and the immunological mechanism of Ru’ai Shuhou Recipe(RSR)in the prevention and treatment of BC.Methods HER2/neu transgenic spontaneous breast tumor model mice were fed with RSR from 5 weeks old,the occurrence of breast tumor in them was observed,and the changes of T cell-mediated immune response and associated cytokines were detected during the carcinogenesis process,i.e.when mice aged between 15 and 25 weeks.Results RSR showed significant effects in postponing and reducing the carcinogenesis of primary breast tumor,up-regulating the amount of T cell in splenic lymphocyte in tumor-bearing mice,promoting the proliferation of T lymphocyte,and inducing the secretion of cytokines such as interleukin-2, interleukin-12 and interferon-γ.Conclusions A serial immune response reveals in the carcinogenesis process. The immunologic function of HER-2/neu transgenic mice is significantly different to that of the same strain nontransgenic mice.Effect of RSR in preventing and postponing breast cancer carcinogenesis is possibly realized through enhancing the anti-tumor immune response of transgenic mice themselves.

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