毒热平注射液体外对小鼠T细胞功能及杀伤FM1感染靶细胞功能的影响

作者	单位
景姗	北京东直门医院消化内科
顾立刚	北京中医药大学中医药抗病毒教育部重点实验室
周芸	北京中医药大学中医药抗病毒教育部重点实验室
李澎涛	北京东直门医院消化内科

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中文摘要:

目的观察毒热平注射液体外对小鼠T细胞功能及杀伤流感病毒亚甲型鼠肺适应株(FM1)感染靶细胞的影响。方法用MTT法及双抗体夹心ELISA法检测毒热平注射液低、中、高(2.1、8.5、17.0μg/mL)剂量对正常小鼠、感染流感病毒亚甲型鼠肺适应株(FM1)的脾细胞数目、产生干扰素-γ(IFN-γ)、白介素-10(IL-10)、及T细胞杀伤FM1感染巨噬细胞Ana-1功能的影响。结果毒热平注射液体外能抑制刀豆蛋白A(Con A)诱导的正常小鼠脾细胞的增殖,抑制Th2型细胞因子IL-10的产生,促进Th1型细胞因子IFN-γ的产生;能保护FM1感染的小鼠脾细胞,并抑制其产生Th2型细胞因子IL-10,而使Th1型细胞因子IFN-γ水平保持在一定的范围内;还能直接增强T细胞对FM1感染的靶细胞(Ana-1)的杀伤能力(P<0.05,P<0.01)。结论毒热平注射液体外作用于小鼠T细胞,可增强有利于抗FM1感染的免疫应答。

英文摘要:

Objective To investigate the effect of Dureping Injection(DRP)on the T-cells function of mice and the function of T-cells in killing MΦinfected by influenza virus subtype A mice-lung adaptive strain FM1 in vitro.Methods Number of splenic normal and FM1 infected T-cells in mice were measured by MTT and double -antibody sandwich ELISA,after being treated with DRP at different concentrations(2.1,8.5 and 17.0μg/mL), and the effect of DRP on interferon-γ(IFN-γ)and interleukin-10(IL-10)production as well as on splenic T-cell killing FM1 infected MΦ/Ana-1 function were detected.Results DRP inhibited the multiplication of normal spleen T cells inducead by concanavalin A in vitro,suppressed Th2 cell factor IL-10 production,and maintained Th1 cell factor IFN-γat a definite level,moreover,it directly enhanced the power of T-cells in killing FM1 infected MΦ(P<0.05,P<0.01).Conclusion DRP could act on mice T-cells to enhance the immune response for antiinfluenza viral FM1 in vitro.

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