人参和三七提取物在血管生成信号通路上作用靶点的实验研究

作者	单位
田伟	中国中医科学院西苑医院中国康复研究中心北京博爱医院中西医结合康复科
雷燕	中国中医科学院西苑医院
杜雪君	中国中医科学院西苑医院
朱凌群	北京中医药大学东直门医院
陈可冀	中国中医科学院西苑医院

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中文摘要:

目的探讨益气活血中药人参和三七提取物在人脐静脉内皮细胞(HUVEC)血管生成信号通路(VEGFR-2-Ras-MAPK)上的作用靶点。方法通过逐级信号阻断的方法,首先加入半数抑制量(IC50)的血管内皮生长因子受体2(VEGFR-2)抑制剂SU5416,采用蛋白免疫印迹(Western blot)法检测其下游信号蛋白Ras、MAPK的表达,其次再加入IC50的Ras抑制剂FPP,采用Western blot检测下游蛋白MAPK的表达。结果与空白对照组比较,加入SU5416后Ras、MAPK的表达均显著下降,差异有统计学意义(P<0.05,P<0.01);加入FPP后MAPK的表达也显著下降,差异有统计学意义(P<0.01)。与SU5416组比较,SU5416加中药组下游信号蛋白Ras、MAPK的表达均显著增加,差异均有统计学意义(P<0.05,P<0.01);与FPP组比较,FPP加中药组下游信号蛋白MAPK的表达显著增加,差异有统计学意义(P<0.05)。结论益气活血中药人参和三七提取物对HUVEC血管生成的作用机制可能是分别促进VEGFR-2、Ras、MAPK这三个关键信号蛋白的表达而实现的。

英文摘要:

Objective To investigate the action targets of ginseng(GS) and Panax notoginseng(PN),Chinese herbs for benefiting qi and activating blood circulation,on angiogenesis signaling pathway(VEGFR-2-Ras-MAPK) in human umbilical vein endothelial cells(HUVEC) .Methods To block the signal pathway by turns,at the first,added the IC50 of VEGFR-2 inhibitor,SU5416,and detected its downstream signaling protein Ras,MAPK expression using Western Blot.Secondly,added the IC50 of the Ras inhibitor,FPP,and detected its downstream signaling protein MAPK expression.Results Compared with the control group,adding SU5416 made the Ras,MAPK expression significantly decreased(P <0.05,P <0.01);and adding FPP made the ex-pression of MAPK significantly decreased(P < 0.01) .Compared with the group treated by SU5416 alone,the expression of downstream signaling protein Ras,MAPK were significantly higher in the group treated by SU5416 plus GS and PN(P <0.05,P <0.01);same state also found in comparison MAPK expression between groups treated with FPP alone and FPP plus GS and PN(P <0.05) .Conclusion The angiogenesis mechanism of GS and PN on HUVEC may be realized by increasing the protein expression of three key signals,VEGFR-2,Ras and MAPK,respectively.

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