青天葵对内毒素致急性肺损伤大鼠肺水通道蛋白1和5表达的影响

作者	单位
许银姬	广东省中医院内三呼吸科
陈远彬	广东省中医院内三呼吸科
王丽丽	广东省中医院内三呼吸科
林琳	广东省中医院内三呼吸科

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中文摘要:

目的通过观察青天葵预先给药对内毒素致急性肺损伤(acute lung injury,ALI)大鼠肺水通道蛋白1和5(aquaporin 1 and 5,AQP-1、AQP-5)表达的影响,探讨其保护作用机制。方法 SD大鼠24只随机分为正常组(A组)、青天葵预保护组(B组)、内毒素模型组(C组)。B、C组采用舌底静脉注射内毒素(5mg/kg)复制ALI模型,B组预先给予10mL/kg青天葵水煎液及每天灌胃处理,造模8h后检测3组肺湿/干重比(W/D),观察肺脏水通透性的变化,HE染色观察肺组织病理学的改变,免疫组化法及逆转录多聚酶链反应(RT-PCR)法检测AQP-1、AQP-5在肺内的表达。结果与C组比较,B组及A组肺W/D比值明显降低(P<0.05),B组肺水肿病理改变程度明显减轻,AQP-1、AQP-5在肺内的蛋白表达水平升高(P<0.05)。结论青天葵预先给药可通过促进ALI大鼠肺AQP-1和AQP-5的表达上调,增加清除和转运肺水,改善水液代谢,减轻肺水肿状态,从而有效保护急性肺损伤。

英文摘要:

Objective To explore the protective mechanism of Nervilia fordii(NF) by observing the effect of its pretreatment on lung aquaporin 1 and 5(AQP-1,AQP-5) expression in rats with endotoxin-induced acute lung injury(ALI) .Methods Twenty-four SD rats were randomly divided into 3 groups,the normal group(A),the NF pre-intervention group(B) and the endotoxin model group(C) .Rats in Group B and C were made into ALI by endotoxin(5 mg/kg) injection via sublingual vein,and NF pretreatment was applied to Group B.Animals were sacrificed at the 8 h after modeling,their lung were taken for observing the water permeability change by wet/dry weight ratio(W/D) measuring,pathological feature by HE staining,and the expression of AQP-1,AQP-5 was detected by immunohistochemistry and RT-PCR.Results The W/D ratio of lung was higher in model rats than in normal rats,but as compared with Group C,it was significantly lower(P <0.05) in Group B.The pulmonary edematous change was significantly mild and the AQP-1 and AQP-5 protein expressions were significantly higher in Group B than in Group C(P <0.05) .Conclusion NF pretreatment can promote lung AQP-1 and AQP-5 expression up-regulation,increase lung water clearance and transportation to improve the water balance and eliminate pulmonary edema,so as to effectively protect lung from acute injury.

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