清胰化积方下调Ski表达抗胰腺癌生长实验研究

作者	单位
王鹏	复旦大学附属肿瘤医院中西医结合科
刘鲁明	复旦大学附属肿瘤医院中西医结合科
陈震	复旦大学附属肿瘤医院中西医结合科
孟志强	复旦大学附属肿瘤医院中西医结合科
林钧华	复旦大学附属肿瘤医院中西医结合科
周振华	复旦大学附属肿瘤医院中西医结合科
陈颢	复旦大学附属肿瘤医院中西医结合科
王琨	复旦大学附属肿瘤医院中西医结合科
沈晔华	复旦大学附属肿瘤医院中西医结合科

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中文摘要:

目的探讨清胰化积方(Qingyi Huaji Formula,QYHJ)是否通过下调Ski表达抑制胰腺肿瘤的生长。方法采用Real-time PCR和Western blot对QYHJ治疗后21天人胰腺癌裸小鼠皮下移植瘤Ski mRNA和蛋白表达进行检测;通过慢病毒介导的RNA干扰技术建立Ski低表达人胰腺癌细胞系,并通过建立裸小鼠皮下移植瘤,评价Ski不同表达肿瘤对QYHJ治疗反应性是否存在差异。结果 QYHJ治疗后,人胰腺癌SW1990皮下移植瘤Ski mRNA和蛋白表达与未用QYHJ治疗组比较分别下降了39.6%和41.3%(均P<0.05);建立了Ski低表达SW1990细胞系(SW1990/Ski RNAi)和阴性干扰细胞系(SW1990/con RNAi),其Ski mRNA和蛋白表达量分别是亲代细胞的105%、123%和46%、30%(均P<0.05);SW1990细胞和SW1990/con RNAi细胞皮下移植瘤应用QYHJ治疗后其瘤重抑制率分别为29.6%和32.2%,但SW1990/RNAi细胞组其瘤重抑制率为16.0%～17.8%(均P<0.05)。结论 Ski下调增加了人胰腺癌细胞对QYHJ治疗的敏感性,Ski为QYHJ治疗胰腺癌一个重要作用靶点,其不同表达介导了胰腺癌细胞对QYHJ治疗的反应性差异。

英文摘要:

Objective To explore the action mechanism of action of Qingyi Huaji Formula(QYHJ), whether its inhibition on the growth of the tumor is by way of down-regulating Ski expression.Methods SW1990 tumor cell with low Ski expression was created by lenti-virus mediated RNA interfering technique.Tumor cells with different levels of Ski expression were subcutaneously transplanted to nude mice,and the response of cancer cells to QYHJ treatment was detected.Results After QYHJ treatment,the tumor growth slowed-down significantly, with the Ski mRNA and protein expressions in tumor reduced by 39.6%and 41.3%of that in untreated tumor respectively(P<0.05).Ski mRNA and protein expressions in the created high and low Ski expression tumor cells were 105%,123%and 46%,30%respectively of that in parental cells(P<0.05).The tumor weight inhibitory rates of QYHJ on high Ski expression cells were 29.6%and 32.2%,while on low Ski expression cells,it was 16.0%to 17.8%(P<0.05).Conclusion Ski acts as an important therapeutic target of QYHJ in treating SW1990 pancreatic cancer,and its expression status mediates different response of tumor cells to QYHJ treatment.

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