人参三七川芎提取物延缓衰老小鼠血管老化的实验研究

作者	单位
雷燕	中国中医科学院西苑医院
杨静	中国中医科学院临床基础医学研究所
赵浩	北京中医药大学大兴中医院
杜雪君	中国中医科学院西苑医院
刘建刚	中国中医科学院西苑医院
陈可冀	中国中医科学院西苑医院

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中文摘要:

目的探讨衰老小鼠血管老化所导致的血管壁结构和功能的改变以及人参、三七、川芎提取物的干预作用。方法建立自然衰老小鼠模型,小鼠随机分为衰老模型组、人参三七川芎提取物高、中、低剂量组、维生素E组,另设青年对照组,观察各组主动脉形态学改变,检测血浆血管紧张素Ⅱ(AngⅡ)、血管组织抗超氧阴离子、晚期糖基化终末产物(AGEs)、基质金属蛋白酶(MMP-2)与其抑制剂(TIMP-2)含量以及MMP-2/TIMP-2比值。结果人参三七川芎提取物能够改善衰老小鼠主动脉形态学衰老变化,HE染色见内皮细胞脱落减少,平滑肌细胞(SMC)增生减少,Masson染色显示中药各组胶原纤维相对含量降低(P<0.05),中药高剂量组SMC相对含量降低(P<0.05)。中药高、中、低剂量组血管组织抗超氧阴离子含量显著增高,AGEs含量明显降低,差异均有统计学意义(P<0.05)。中药各剂量组小鼠血管组织MMP-2水平降低,TIMP-2水平显著升高,MMP-2/TIMP-2比值明显增高,差异均有统计学意义(P<0.05)。结论人参三七川芎提取物能够改善衰老小鼠主动脉形态学衰老变化,降低血管组织中活性氧的生成,减少AGEs生成,抑制MMP-2的活性,调节MMP-2/TIMP-2的平衡,从而最终降低衰老小鼠血管的僵硬度,减少血管重构,延缓小鼠血管老化的发生。

英文摘要:

Objective To explore the vascular structural and functional change in aged mice,and the intervention roles of extracts from ginseng,notoginseng and chuanxiong(Exs) on it.Methods The mice model of natural aging was built,and all model mice were divided into 5 groups,the model group,the Vitamin E group treated with vitamin E,and the three Exs groups treated with high,middle and low dose Exs respectively.Besides, a normal control group was set up with young rats.Morphological change of aorta was observed by HE and Masson staining,levels of plasma angiotensin-Ⅱ(AngⅡ),anti-superoxide anion,advanced glycation end products(AGEs),matrix metalloproteinase 2(MMP-2) and tissue inhibitor of metalloproteinase 2(TIMP-2) in vascular tissue,as well as the MMP-2/TIMP-2 were detected.Results Aged aortic morphologic change in model rats was ameliorated in the Exs treated groups,decreased vascular endothelial exfoliative cells and vascular smooth muscle cell(SMC) proliferation were shown in HE staining.Masson staining analysis showed relative content of collagen fibers reduced in all Exs treated groups(P<0.05) and that of SMC decreased in high-dose Exs group(P<0.05).Moreover,levels of vascular tissue anti-superoxide anion,TIMP-2 and MMP-2/TIMP-2 ratio were obviously higher,AGEs and MMP-2 were significantly lower in all Exs treated groups than those in the model group respectively,showing statistical significance(P<0.05).Conclusion Exs can ameliorate the aged changes in aortic morphology,reduce the production of reactive oxygen species(ROS) and AGEs in vascular tissue,inhibit MMP-2 activity and regulate MMP-2/TIMP-2 equilibrium,so it reduces the vascular stiffness degree of senescent mice,decreases vascular remodeling and delays the occurrence of vascular aging ultimately.

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