蝙蝠蛾拟青霉Cs-4对阿霉素肾病大鼠的保护作用

作者	单位
王娜	山东大学齐鲁医院肾内科
亓敏	山东大学齐鲁医院肾内科
梁素忍	山东大学齐鲁医院肾内科
甄军晖	山东大学齐鲁医院肾内科
刘春喜	山东大学齐鲁医院肾内科
胡昭	山东大学齐鲁医院肾内科

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中文摘要:

目的研究蝙蝠蛾拟青霉Cs-4对阿霉素肾病大鼠的保护作用。方法雄性SD大鼠24只,随机分为正常组、模型组、蝙蝠蛾拟青霉Cs-4组、贝那普利组。采用左侧肾脏切除加尾静脉重复注射阿霉素的方法建立阿霉素肾病模型。术后1周蝙蝠蛾拟青霉Cs-4组给予蝙蝠蛾拟青霉Cs-4 500 mg/(kg·d)灌胃,贝那普利组予贝那普利4 mg/(kg·d)灌胃。用药4、8周后观察各组大鼠24 h尿蛋白定量、血生化(BUN、Scr),8周后取肾组织于光镜、电镜下观察肾脏病理变化,应用免疫组化方法测定肾组织骨调素(OPN)、纤维连接蛋白(FN)、胶原Ⅳ(COL-Ⅳ)的表达。结果蝙蝠蛾拟青霉Cs-4能减少阿霉素肾病大鼠尿蛋白,延缓BUN、Scr的上升,纠正脂代谢紊乱,与模型组相比差异显著(P<0.05);减轻肾小球系膜基质比(P<0.05),减少OPN、FN、COL-IV的表达(P<0.05)。结论蝙蝠蛾拟青霉Cs-4可减少蛋白尿的排泄,纠正脂代谢紊乱,改善肾功能,抑制ECM的过度积聚,从而减轻肾脏病理损害,发挥其肾脏保护作用。

英文摘要:

Objective To study the renal protective effect of Paecilomyces hepiali Cs-4(Abbr,to Cs-4) on rats with adriamycin-induced nephropathy(AIN).Methods Twenty-four male SD rats were randomly divided into four groups:the control group(A),the model group(B),the Benazepril group(C),and the Cs-4 group (D).AIN rat model was established by left unilateral nephrectomy and repeated caudal vein injection of adriamycin. Gastric perfusion of Cs-4[500 mg/(kg·d)]and Benazepril[4 mg/(kg·d)]was given respectively to the groups D and C,starting from 1 week after modeling.Blood biochemical indices including blood urea nitrogeni (BUN),serum creatine(Scr) and 24-hour urinary protein excretion(24 h-UP) were assessed 4 and 8 weeks after medication.Rats were sacrificed at the end of 8-week medication,their renal tissue was get for pathological examination with electric microscopy,the expressions of fibronection(FN),collagenⅣ(COL-Ⅳ) and osteopont (OPN) in renal tissue were assessed by immunohistochemistry.Results Levels of 24h-UP,BUN,Scr, mesengial matrix percentage,as well OPN,FN,COL-Ⅳwere significantly lower in group D than in group B(P<0.05);and the fat metabolic disorder was improved in group D(P<0.05).Conclusion Cs-4 displays its renal protective action in rats with AIN through reducing the urinary protein excretion,correcting lipid metabolic disturbance, inhibiting the over accumulation of extracellular matrix,improving the pathological damage of kidney, thus restoring the renal function.

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