大脑中动脉栓塞大鼠脑区病理生理动态变化及不同频率针刺法的影响

Dynamic Physiologic and Pathologic Changes in Brain of Rat with Middle Cerebral Artery Obstruction and Effects of Acupuncture in Different Frequencies on Them

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中文关键词: 大脑中动脉栓塞病理生理针刺方法针刺频率

英文关键词:middle cerebral artery obstruction physiology and pathology method of acupuncture fre- quency of acupuncture

基金项目:国家重点基础研究发展计划(973计划)资助项目(No.2006CB504500,No.2006CB504504);天津市科技发展计划资助项目(No.043609811);天津市高等学校科技发展基金资助项目(No.20030113,No.20050319)

作者	单位
张雪	内蒙古包头医学院第二附属医院针灸科
樊小农	天津中医药大学第一附属医院天津市针灸研究所天津市针灸学重点实验室
王舒	天津中医药大学第一附属医院天津市针灸研究所天津市针灸学重点实验室
钱宇斐	天津中医药大学
张亚男	天津中医药大学
魏媛媛	天津中医药大学
武慧群	天津中医药大学
卢红朵	天津中医药大学
孙红红	天津中医药大学
郭海燕	天津中医药大学
李凌鑫	天津中医药大学
石学敏	天津市针灸研究所天津市针灸学重点实验室

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中文摘要:

目的了解大脑中动脉栓塞(middle cerebral artery obstruction,MCAO)大鼠不同脑区微血管、炎性细胞的动态变化及毫针针刺法中频率因素对其影响。方法参照Zea-Longa线拴法复制大鼠MCAO模型,对"醒脑开窍"针刺法主穴水沟施以快频率180次/s和慢频率60次/s,持续时间均为5 s的针刺干预,共6次,计数皮层、海马、纹状体部位微血管及炎性细胞的变化。结果模型组各部位(除纹状体)的炎性细胞数和微血管数无明显变化;造模72 h后,未针刺组与正常组比较,纹状体的微血管数明显减少,皮层和海马的炎性细胞数明显增多;与未针刺组比较,快频率针刺组可明显减少皮层和海马的炎性细胞数、慢频率针刺组可明显增加纹状体的炎性细胞数和微血管数,各组比较差异有统计学意义(P<0.05)。结论 MCAO大鼠在脑缺血后,会随着时间变化出现微血管和炎性细胞动态病理变化并表现出明显的脑区特异性;快、慢频率针刺法改善脑缺血的作用环节不同,同样具有脑区部位的特异性。

英文摘要:

Objective To study the dynamic changes of capillaries and inflammatory cells in different regions of brain in rat with middle cerebral artery obstruction(MCAO),and the effects of acupuncture in different frequencies on them.Methods In reference to Zea-Longa’s method,rat model of MCAO was established by thread-ligation.Shuigou point(DU26),the main acupoint for "awakening brain and opening apertures",was stimulated by high(180 times/s) or low(60 times/s) frequency puncturing 5 s every 12 h for 6 times totally. The amount of capillaries(AC) and inflammatory cells(AIC) in brain cortex(BC),hippocampus(Hp) and corpus striatum(CS) was counted.Results Changes in AC and AIC of all brain regions(except for CS) in rats immediately after modeling were statistically insignificant(P>0.05).But 72 h later,AC in CS decreased,AC in Hp,AIC in BC and AIC in Hp increased significantly in the modeled rats,showing significant difference to the normal level,but AIC reduced to approach the normal.As compared with the rats un-intervened,AIC in BC and Hp was decreased in rats intervened with high frequency puncturing,AC and AIC in CS were increased in rats intervened by slow frequency puncturing(both P<0.05).Conclusion Amount of capillaries and inflammation cells are changed dynamically in MCAO rats after brain ischemia,showing evident brain regional specificity;the ischemic improving effects of acupuncture in different frequencies are various in their action rings,also showing brain regional specificity.

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