| 张海啸,杨叔禹,曹洪欣,鞠大宏,潘静华,李玉梅,张立石.平糖方药物血清改善INS-1胰腺β细胞脂性凋亡的作用研究[J].中国中西医结合杂志,2010,30(9):978-981 |
| 平糖方药物血清改善INS-1胰腺β细胞脂性凋亡的作用研究 |
| Effect of Pingtang Recipe Containing Drug-Serum on INS-1 Pancreatic β Cells Lipoapoptosis |
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| DOI： |
| 中文关键词: 平糖方 药物血清 INS-1胰腺β细胞 脂性凋亡 活性氧 解偶联蛋白-2 |
| 英文关键词:Pingtang Recipe drug-serum INS-1βpancreatic cells lipoapoptosis reactive oxygen species uncoupling protein-2 |
| 基金项目:中国博士后科学基金资助项目(No.20090450832);福建省自然科学基金;国家自然科学基金资助项目(No.30973912) |
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| 中文摘要: |
| 目的观察平糖方药物血清对INS-1胰腺β细胞脂性凋亡的改善作用及机制。方法以软脂酸(palmitic acid,PA)诱导INS-1胰腺β细胞脂性凋亡,采用平糖方药物血清干预。实验分为5组,即大鼠血清对照(RS)组、大鼠血清加PA(PA)组、平糖方低剂量药物血清加PA(PTR L)组、平糖方中剂量药物血清加PA(PTR M)组、平糖方高剂量药物血清加PA(PTR H)组,每组6个复孔。通过TUNEL法检测细胞凋亡,化学发光法检测Caspase-3活性,DCHF-DA掺入法检测细胞内ROS生成的变化,RT-PCR方法检测解偶联蛋白-2(UCP-2)的表达。结果 PA组比RS组的凋亡细胞增多(P<0.01),PTR各组的凋亡细胞同PA组比较有降低的趋势。PA组INS-1胰腺β细胞Caspase-3酶活性增强,PTR M组同PA组比较Caspase-3酶活性降低(P<0.05)。PTR L、M组可抑制由PA引起的细胞内ROS产生增多,与PA组比较差异有统计学意义(P<0.05)。平糖方各组的UCP-2 mRNA表达量均较PA组降低(其中PTR L 1.286±0.373,P<0.01;PTRM 1.627±0.348,P<0.05)。结论平糖方药物血清对INS-1β细胞脂性凋亡有一定的保护作用,并可能通过调节ROS、UCP-2发挥作用。 |
| 英文摘要: |
| Objective To observe the effect and mechanism of Pingtang Recipe containing drug-serum (DS-PTR) in improving INS-1βpancreatic cells lipoapoptosis.Methods Experimental INS-1βcells were divided into 5 groups(6 pools for each group),namely,the blank control group treated with rat’s serum(C),the other 4 model groups induced into lipoapoptosis by palmitic acid and treated respectively by rat’s serum(M), high,middle and low dose DS-PTR(DSh,DSm and DSI).Cell apoptosis was detected by TUNEL staining; Caspase-3 activity of cells was measured by chemiluminescence method;intracellular production of reactive oxygen species(ROS) was detected by DCHF-DA incorporation,and expressions of uncoupling protein-2(UCP-2) was determined by RT-PCR.Results INS-1βcell apoptosis in Group M was significantly higher than that in Group C(P<0.01),while that showed a decreased trend in the three DS-PTR treated groups.Caspase-3 activity was enhanced in Group M,it decreased significantly in Group DSm(P<0.05).The over-produced ROS in cells after modeling was inhibited in Groups DSm and DSI(P<0.05),meantime,expression of UCP-2 excited by PA(2.244±0.421 ) was reduced significantly in Group DSI and Group DSm to 1.286±0.373(P<0.01 ) and 1.627±0.348(P<0.05) respectively.Conclusion DS-PTR shows a protective effect on INS-1βpancrentic cells against lipoapoptosis,which is possibly play its mechanism through regulating ROS and UCP-2. |
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