半夏白术天麻汤对自发性高血压大鼠左心室肥厚的影响

作者	单位
蒋嘉烨	上海中医药大学教学实验中心
王现珍	上海中医药大学教学实验中心
罗珊珊	上海中医药大学教学实验中心
王勋	上海中医药大学教学实验中心
卞卡	上海中医药大学教学实验中心
可燕	上海中医药大学教学实验中心

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中文摘要:

目的探讨自发性高血压大鼠(spontaneous hypertensive rat,SHR)肥厚心肌肾素-血管紧张素系统(renin angiotensin system,RAS)的改变,并观察不同时期半夏白术天麻汤对其血流动力学和RAS中各因子mRNA的影响。方法将54只6周龄SHR分为3组:半夏白术天麻汤治疗组、卡托普利对照组及SHR组,每组18只;药物分别溶于蒸馏水中,大鼠灌胃至24周龄,停药后继续观察到32周龄。SHR组和血压正常的Wistar-Kyoto大鼠(Wistar-Kyoto rats,WKY)灌服等量的蒸馏水,各组在18、24和32周龄时分3批处理。处理期间颈动脉插管测量血流动力学指标,观察病理学切片,Real-time PCR检测血管紧张素原(angiotensin,AGT)、血管紧张素转换酶(angiotensin-converting,ACE)、血管紧张素转换酶2(angiotensin-converting2,ACE2)mRNA的表达。结果与WKY组比较,同周龄SHR在18周龄、24周龄和32周龄时动脉压和左室质量指数均显著升高(P<0.01),血流量差异无统计学意义,心电图平均周期速率较高(P<0.05),AGT和ACE mRNA表达均较高(P<0.01),而在18周龄、24周龄时ACE2mRNA表达较低(P<0.01)。半夏白术天麻汤组在18周龄和24周龄时均可显著降低动脉压(P<0.05),改善心肌结构,在24周龄时显著降低左室质量指数(P<0.05),在18、24和32周龄时AGT、ACE显著降低和ACE2mRNA的表达显著升高(P<0.05)。结论 SHR肥厚心肌中RAS发生变化,这可能是导致高血压左心室肥厚的分子机制之一。应用半夏白术天麻汤可改善血流动力学指标,调节RAS进而降低动脉压。

英文摘要:

Objective To investigate the changes in renin angiotensin system(RAS)in hypertrophied myocardium of spontaneous hypertensive rat(SHR),and the effect of Banxia Baizhu Tianma Decoction(BBTD)on the changes in haemodynamic parameters and mRNA of signaling molecules of RAS at different periods.Methods Fifty-four male SHRs of 6 weeks old were randomly and equally divided into three groups :the untreated control group,the test group,and the positive control group,and they were treated respectively with distilled water,BBTD and captopril by dissolving in equal volume of water administrated via gavage for 18 weeks.Besides,18 age matched Wistar-Kyoto(WKY)rats treated with distilled water were allocated in a normal control group.Rats were managed in batches at their age of 18,24,and 32 weeks old.Rat’s hemodynamic parameters were measured through carotid artery catheterization,myocardial pathology was observed,and their mRNA expressions of angiotensin(AGT),angiotensin-converting(ACE)and angiotension-converting 2(ACE2)were determined by Real-time PCR.Results Compared with WKY rats,the arterial pressure and left ventricular mass index(LVMI)in SHR were significantly higher at 18,24 and 32 weeks respectively(P<0.01);average cycle rate showed in electrocardiogram was higher(P<0.05),though the blood stream was similar;mRNA expressions of AGT and ACE in heart tissue were markedly higher(P<0.01),but that of ACE2 at 18 and 24 weeks were lower(P<0.01).Compared with untreated SHR,arterial pressure at 18 and 24 weeks was lower(P<0.05);cardiac muscle structure was improved;LVMI at 24 weeks was improved(P<0.05);the mRNA expressions of AGT and ACE were suppressed but that of ACE2 increased at 18,24,and 32 weeks significantly in the test group after BBTD treatment(P<0.05).Conclusions Changes in RAS in the hypertrophied myocardium of SHR may be one of the molecular mechanisms for hypertension leading to left ventricular hypertrophy.BBTD can improve the hemodynamic parameters,regulate RAS,so as to lower the arterial pressure.

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