从代谢组学的角度探讨维胃方对大鼠实验性胃溃疡的疗效机制

作者	单位
彭树灵	南方医科大学中医药学院中医诊断教研室
刘晓伟	南方医科大学中医药学院中医诊断教研室
张真瑞	南方医科大学中医药学院中医诊断教研室
杨健	南方医科大学中医药学院中医诊断教研室
贺红燕	南方医科大学中医药学院中医诊断教研室
肖卫平	南方医科大学中医药学院中医诊断教研室

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中文摘要:

目的从代谢组学的角度探讨维胃方对大鼠实验性胃溃疡的疗效机制。方法采用乙酸灼烧法制备大鼠胃溃疡模型;采用气相色谱-质谱联用仪(GC-MS)获得正常对照组、模型组、自愈组与维胃方治疗组的大鼠胃黏膜匀浆代谢物谱。经数据前处理后,进行偏最小二乘法判别分析(PLS-DA),寻找表征正常对照组与模型组差异的生物标记物;使用主成分分析(PCA)考察维胃方对胃溃疡的治疗效果,并根据生物标记物的变化解释其作用机制。结果模型组与正常对照组的胃黏膜代谢物谱差异有统计学意义;生物标记物包括有机酸、脂肪酸、氨基酸等物质,表明胃溃疡大鼠的胃黏膜出现能量代谢与物质代谢异常;维胃方通过调节胃黏膜的代谢功能,达到有效治疗胃溃疡的目的。结论运用代谢组学技术从整体上揭示了维胃方对大鼠实验性胃溃疡的疗效机制,凸显了中药多靶点综合治疗的特色。

英文摘要:

Objective To investigate the therapeutic mechanism of Weiweifang(WWF,a Chinese herbal preparation)on gastric ulcer in rats viewing from metabonomics.Methods Wistar rats were made to gastric model by acetic acid cauterization and randomized into the model group,the spontaneously healing group and the three WWF treatment groups,and a group of normal rats was set for control.Metabolic spectra of gastric mucosa extraction of rats were acquired with gas chromatography-mass spectrometry(GC-MS)technique.After being pre-processing,data were subjected to partial least squares discriminant analysis(PLS-DA)to discover the biomarkers in rats of the normal group and the model group.The therapeutic effect of WWF on experimental gastric ulcer was assessed by principal component analyses(PCA),and its action of mechanism was explained viewing from the changes of biomarkers.Results Spectra of biomarkers,including organic acids,fatty acids,amino acids,etc.in model rats were statistically different to those in normal rats,which demonstrated that the energy and substance metabolisms were disordered in rats with gastric ulcer.WWF could cure gastric ulcer effectively by way of regulating the metabolism of gastric mucosa.Conclusion The therapeutic mechanism of WWF on experimental gastric ulcer in rats is revealed integrally by metabonomics in this study,displaying prominently the characteristics of Chinese medicine multiple targets comprehensive therapy.

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