黄芪总苷对大鼠神经元缺氧/复氧损伤的影响

作者	单位
尹艳艳	安徽医科大学药理学教研室
朱芬芳	安徽医科大学药理学教研室
吴国翠	安徽医科大学药理学教研室
李维祖	安徽医科大学药理学教研室
公惠玲	安徽医科大学药理学教研室
李卫平	安徽医科大学药理学教研室

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中文摘要:

目的探讨黄芪总苷(astragalosides,AST)对大鼠神经元缺氧/复氧损伤的影响。方法建立原代培养的大鼠海马神经元的缺氧/复氧模型。采用四唑蓝(MTT)法和乳酸脱氢酶(LDH)释放法检测细胞活力;检测细胞上清液中丙二醛(MDA)和一氧化氮(NO)含量、超氧化物歧化酶(SOD)活性;Hoechst染色和AnnexinV/PI双染流式细胞术检测细胞凋亡;采用激光共聚焦显微镜和Ca2+荧光探针Fluo-3/AM观察细胞内游离钙[Ca2+]i。结果黄芪总苷可以提高缺氧/复氧后神经元的活力,降低细胞上清液中MDA和NO含量,提高SOD活性,降低细胞凋亡率,减轻神经元内的钙超载。结论黄芪总苷对缺氧/复氧损伤的神经元具有保护作用,其机制可能与抗氧化和减轻钙超载有关。

英文摘要:

Objective To study the effect of astragalosides(AST) on the anoxia/reoxygenation(A/R) injured neuron in rat.Methods Primary cultured rat’s hippocampal neurons were made into A/R model cells.The cell viability was detected by MTT assay and lactate dehydrogenase releasing methods;the activity of superoxide dismutase(SOD),and contents of malondialdehyde(MDA) and nitride oxide(NO) in culture supernate were detected;the apoptosis rate of hippocampal neurons after A/R was measured by flow cytometry with double-staining of hoechst33258 and AnnexinVPI;and intracellular calcium ion [Ca2+]i was observed with a cofocal laser-scanning microscope and determined by fluorescent probe Fluo-3/AM.Results AST enhanced the cell viability of neurons after A/R injury,increased SOD activity and decreased the MDA and NO contents in supernate,reduced the A/R-induced apoptosis and decreased the calcium overload in neurons.Conclusion AST has the protective effects on A/R injured neurons,the mechanism is possibly related with its anti-oxidation and calcium overload reducing actions.

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