红景天与熟地配伍对骨髓抑制小鼠骨髓功能的影响

目的探讨红景天与熟地配伍对环磷酰胺所致小鼠外周血白细胞下降及骨髓造血功能抑制的影响。方法 ICR小鼠腹腔注射环磷酰胺致化学损伤骨髓抑制模型,连续灌胃给药10天,观察红景天和熟地单味药、红景天熟地合剂、红景天苷、红景天苷加熟地合剂对外周血象、骨髓有核细胞增殖的影响,对骨髓粒-巨噬系祖细胞(CFU-GM)集落形成、集落刺激因子(GM-CSF)及促红细胞生成素(EPO)分泌的影响。结果红景天熟地合剂组及红景天苷加熟地组对骨髓抑制模型小鼠外周血白细胞具有明显增高作用;红景天熟地合剂组对骨髓有核细胞下降具有拮抗作用,可明显提高CFU-GM集落生成,与模型组比较,差异有统计学意义(P<0.01);红景天熟地合剂可明显提高GM-CSF的产生,与模型组比较差异具有统计学意义,对EPO产生略有提高,但差异无统计学意义。结论红景天熟地合剂通过促进骨髓细胞与集落的产生,及GM-CSF等细胞因子的合成,对造血系统进行调控,增加外周血白细胞数量,恢复机体造血机能。

英文摘要:

Objective To explore the effect of combined use of rehmannia(RM) and rhodiola(RD) on peripheral leukopenia and bone marrow hematopoietic function suppression induced by cyclophosphamide(CTX) in mice.Methods ICR mice were established into bone marrow inhibition models by intraperitoneal injection of CTX,and were administered with RM,RD or its extract(RDE),singly or in mixture,via gastrogavage for 10 days.The changes of peripheral hemogram,bone marrow nucleated cell proliferation,CFU-GM colony formation,GM-CSF and erythropoietin(EPO) secretion were observed.Results Compared with the un-treated model mice,the peripheral white blood cell count was significantly higher in model mice treated with RDE and RM mixture;the bone marrow nucleated cells count,CFU-GM formation,and GM-CSF production were significant higher in model mice treated with RD and RM mixture,showing statistical significance(P<0.01);while EPO production in the RD and RM mixture treated group was slightly elevated,but the difference showed no statistical significance.Conclusion RD and RM mixture could regulate hematopoietic system by promoting the production of bone marrow cells and colonies,as well as enhancing the synthesis of related cytokines,such as GM-CSF,so as to increase the amount of peripheral white blood cells and restore the hematopoietic function of organism.

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