交泰丸及其单味药对2型糖尿病大鼠异位脂肪沉积的影响

作者	单位
龚艳琳	华中科技大学同济医学院附属同济医院中西医结合研究所
陆付耳	华中科技大学同济医学院附属同济医院中西医结合研究所
董慧	华中科技大学同济医学院附属同济医院中西医结合研究所
徐丽君	华中科技大学同济医学院附属同济医院中西医结合研究所
汪健红	华中科技大学同济医学院附属同济医院中西医结合研究所
邹欣	华中科技大学同济医学院附属同济医院中西医结合研究所

摘要点击次数: 1296

全文下载次数: 777

中文摘要:

目的探讨交泰丸及其单味药对2型糖尿病(T2DM)大鼠异位脂肪沉积的影响。方法采用尾静脉注射小剂量链脲佐菌素(STZ)加高脂高热卡喂养的方法建立大鼠T2DM模型,将大鼠随机分为模型组、交泰丸组、黄连组、肉桂组、二甲双胍组,各治疗组分别给予相应药物灌胃治疗,另设正常对照组。干预治疗8周后检测各组大鼠体重、肝指数、空腹血糖、空腹胰岛素、胰岛素抵抗指数(HOMA-IR)、血浆肝酶活性等指标,以及各组大鼠肝脏、心肌、骨骼肌组织甘油三酯(TG)含量和病理学改变。结果与正常组比较,模型组大鼠体重和肝指数增加,肝酶活性升高,HOMA-IR增加,肝脏、心肌、骨骼肌组织TG含量升高(P﹤0.05或P﹤0.01),病理学观察均有不同程度的脂肪变性;与模型组比较,各治疗组大鼠肝指数下降,肝酶活性降低,HOMA-IR下降,肝脏、心肌、骨骼肌组织TG含量均有不同程度降低(P﹤0.05或P﹤0.01),组织学接近正常;与二甲双胍组比较,交泰丸组肝脏、骨骼肌TG含量明显降低(P﹤0.01),黄连组骨骼肌TG含量明显降低(P﹤0.05);各中药组比较,交泰丸组降低γ-谷氨酰氨基转肽酶(γ-GT)功效为最佳(P﹤0.01)。结论交泰丸及其单味药可改善T2DM大鼠糖脂代谢紊乱和肝功能异常,其机制可能与改善胰岛素抵抗和减少异位脂肪沉积有关。

英文摘要:

Objective To study the effects of Jiaotai Pill (JTP) and its single components on ectopic fat accumulation in rats with type 2 diabetes mellitus (T2DM).Methods The T2DM model of rat was established by injection of streptozotocin from tail vein and high fat-caloric diet feeding.Model rats were randomly divided into the model group and four treated groups were treated respectively with JTP and its single components,Rhizoma Coptidis,Cinnamon and metformin,via gastric perfusion.Meanwhile,a normal control group was also set up.Body weight (BW),liver index (LI),levels of fasting plasma glucose (FPG),fasting serum insulin (FINS) and insulin resistance index (HOMA-IR),plasma activities of liver associated enzymes (LAE),triglyceride (TG) contents and pathological changes of liver,heart and muscle were determined before and after a 8-week treatment.Results As compared with the normal rats,BW,LI,LAE activities,HOMA-IR,TG contents of the liver,heart and muscle were all increased in the model rats (P<0.05 or P<0.01),with pathologic appearance of fatty degeneration in different degrees.Compared with the model group,LI,LAE,HOMAIR,and TG contents in the liver,heart and muscle tissues were decreased in different extents in the four treated groups (P<0.05 or P<0.01),and the histology of tissues in them was restored to near normal.Compared with the metformin treated group,the hepatic and muscular TG contents decreased in the JTP treated group (P<0.01),and the muscular TG content in the Rhizoma Coptidis treated group were lower (P<0.05).And the γ-GT level in the JTP treated group was the lowest in the three Chinese drugs treated groups (P<0.01).Conclusions The disturbances of glucose and lipid metabolism and abnormality of liver function in T2DM rats could be improved by JTP and its single components.The mechanism might be related to their effects in improving insulin resistance and reducing ectopic fat accumulation.

关闭