黄芩苷对脑出血大鼠脑组织凝血酶受体-1表达及细胞凋亡的影响

目的研究黄芩苷对大鼠脑出血后神经组织的保护作用及其机制。方法大鼠随机分为5组:假手术组、脑出血模型组、黄芩苷小、中、大剂量组,采用胶原酶Ⅶ诱导大鼠脑出血模型,术后2h各治疗组给予不同剂量的黄芩苷腹腔注射,余组给予等容量生理盐水,每天1次,连续用药1、3、5、10天后取脑组织,Western blot法检测脑出血周围脑组织凝血酶受体1(protease-activated receptor-1,PAR-1)表达,TUNEL法检测细胞凋亡情况,干湿比重法测定脑组织水肿情况。结果与脑出血模型组比较,黄芩苷各治疗组的大鼠脑组织PAR-1表达和TUNEL阳性细胞数明显减少、脑水肿减轻,差异均有统计学意义(P<0.01)。结论脑出血后的脑组织PAR-1高表达可能介导了细胞凋亡和脑水肿,黄芩苷对脑出血大鼠有保护作用,可能参与抑制脑出血后脑组织PAR-1表达、减少细胞凋亡,从而减轻脑水肿症状。

英文摘要:

Objective To investigate the protective effect and mechanism of baicalin on nerve tissue in rat with intracerebral hemorrhage (ICH).Methods Rats were randomly divided into five groups:the sham-operated group,the ICH model group,and the three baicalin treated groups treated respectively with small,medium and large doses of baicalin.ICH rat model was established by injecting collagenase Ⅶ into caudate nucleus.Baicalin was given by peritoneal injection to the baicalin treated groups,and saline was given to the other two groups once a day started from 2h after modeling.Animals were sacrificed in batches on the 1st,3rd,5th and 10th day of treatment to take their brains for detecting protease-activated receptor-1 (PAR-1) expression and cell apoptosis in brain tissue surrounding hematoma by Western blot and TUNEL method,respectively.And the water content of brain was estimated by drywet weight method.Results Compared with the model group,the PAR-1 expression and TUNEL-positive cells were significantly reduced in the baicalin treated groups;and brain edema was also significantly reduced (P<0.01).Conclusions The up-regulated PAR-1 expression after ICH in rats might play an important role in inducing cell apoptosis and brain edema.Baicalin shows significant protective effect on ICH rats,which may be related to its effects in inhibiting PAR-1 expression and decreasing apoptosis cells,so as to reduce brain edema.

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