气郁体质相关差异基因研究

目的探讨气郁体质者与非气郁体质者的相关差异基因,从而运用分子生物学的手段揭示气郁体质的遗传本质。方法应用mRNA差异显示技术逆转录-聚合酶链反应(differential display reverse tran-scription polymerase chain reaction,DD-RT-PCR),对通过气郁体质判定标准的气郁体质者3例和通过平和质体质类型论述并结合专家判别的非气郁体质者2例,进行两者之间初步的差异基因的研究。结果在气郁质组和非气郁质组的大部分基因表达是相同的,一部分基因表达在非气郁质组中不存在,而一部分在气郁质组中呈高表达,包括丛状蛋白A2与人染色体1q32有98%同源性,notch前蛋白原与人染色体1q11有98%同源性,核纤层蛋白A基因与人染色体17q25有100%同源性,T细胞受体δ链基因与人染色体1q24有99%同源性,神经细胞PAS域蛋白3与人染色体14q13有98%同源性,S-相位激酶相关蛋白2基因与人染色体5p12有99%同源性。结论气郁体质与非气郁体质之间基因可能存在差异性。

英文摘要:

Objective To investigate the differential genes between the qi-stagnation constitution (QSC) and non-QSC,for the sake of disclose the hereditary essence of QSC. Methods The constitution of 3 patients was judged as QSC by criteria for QSC and that of 2 patients was determined as non-QSC by experts according to the gentle constitution related treatise. The differential genes between them were studied using the mRNA differential display revers transcription polymerase chain reaction(DD-RT-PCR). Results Some differential genes were found (part of them not found in non-QSC,and part was highly expressed in QSC). Among the differential genes,homology was shown between plexin A2-human chromosomal 1q32 (in 98%),notch pre-proteinogen-chromosomal 1q11 (98%),nuclear prelamin A-chromosomal 17q25 (100%),T-cell receptor δ chain-chromosomal 1q24 (99%),neuronal PAS domain protein 3-chromosomal 14q13 (98%),S-phase kinase-associated protein 2-chromosomal 5p12 (99%). Conclusions Some differential genes may be existed between QSC and non-QSC.

关闭