补肾调经方、逍遥丸对促性腺激素预处理小鼠组织蛋白酶-L mRNA的影响

作者	单位
宋翠淼	河北医科大学生理教研室
贺明	河北医科大学中西医结合学院
段彦苍	河北医科大学中西医结合学院
杜惠兰	河北医科大学中西医结合学院

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中文摘要:

目的探讨补肾调经方、逍遥丸对小鼠组织蛋白酶-L(cathepsin-L,Cat L)mRNA的影响。方法选择未成年小鼠随机分为正常组、对照组、逍遥丸组和补肾调经方组,每组12只,采用反转录聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)方法检测给予5IU人绒毛促性腺激素(humanchorionic gonadotropin,HCG)0、4、8、12h后Cat L mRNA在各组小鼠卵巢的表达强度。结果注射HCG0、4、8、12h后,Cat L mRNA在促性腺激素预处理小鼠对照组卵巢的表达逐渐升高,Cat L mRNA的相对表达含量分别为:0.066±0.005、0.383±0.045、0.737±0.024、1.036±0.073,各时间点比较差异有统计学意义(P<0.01)。注射HCG后4h与对照组比较,Cat L mRNA逍遥丸组、补肾调经方组小鼠卵巢表达均升高,差异有统计学意义(P<0.01);注射HCG8h与对照组比较,逍遥丸组Cat LmRNA表达升高,差异有统计学意义(P<0.01),补肾调经方组差异无统计学意义(P>0.05);注射HCG12h后与对照组比较,补肾调经方组Cat LmRNA表达升高,差异有统计学意义(P<0.01),逍遥丸组差异无统计学意义(P>0.05)。结论补肾调经方通过使Cat L mRNA表达增强而达到促排卵的作用,消遥丸通过使Cat L mRNA表达高峰提前达到促排卵的目的。

英文摘要:

Objective To study the effect of Bushen Tiaojing Recipe (BTR) and Xiaoyao Pill (XP)on cathepsin-L (Cat-L) mRNA in mice. Methods Immature mice were randomly divided into the normal group,the control group,the BTR group and the XP group,three in each group. Cat-L mRNA expression in mice was detected using reverse transcription polymerase chain reaction (RT-PCR)at 0,4,8 and 12 h after injecting 5 IU(human chorionic gonadotropin,HCG). Results Cat-L mRNA expression increased gradually after HCG injection,the relative levels in the control group at 0,4,8 and 12 h were 0.066±0.005,0.383±0.045,0.737±0.024 and 1.036±0.073 respectively,comparisons between different time-points showed significant difference (P<0.01). Compared with the control group,the Cat L mRNA expression was higher at 4 h in both BTR and XP groups(P<0.01),at 8 h in the XP group (P<0.05),and at 12 h in BTR group after injecting HCG (P<0.05).Compared with the control group,cat L mRNA expression showed no statistic difference at 8 h in BTR group and at 12 h in XC group. Conclusions BTR promoted the ovulation by enhancing the expression of CatL gene,and that of XP by advancing the peak of CatL gene expression.

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