益气除痰方对脾虚Lewis肺癌小鼠肿瘤生长、生存期及PRDX-1、PRDX-6表达的影响

Effects of Yiqi Chutan Recipe on Tumor Growth,Survival Time and Expressions of PRDX-1 and PRDX-6 in Lewis Lung Carcinoma Model Mice with Pi-deficiency Syndrome

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中文关键词: 益气除痰方脾虚证 Lewis肺癌生存期过氧化物酶-1 过氧化物酶-6

英文关键词:Yiqi Chutan Recipe Pi-deficiency syndrome Lewis lung carcinoma survival time peroxiredoxin-1 peroxiredoxin-6

基金项目:国家自然科学基金资助项目(No.30772862)

作者	单位
周京旭	广州中医药大学第一临床学院肿瘤科
王淑美	重庆医科大学中医药学院
林丽珠	广州中医药大学第一临床学院肿瘤科

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中文摘要:

目的观察益气除痰方对脾虚C57BL/6J小鼠Lewis肺癌肿瘤生长、肺转移、生存期及肿瘤组织中过氧化物氧化还原酶PRDX-1、PRDX-6表达的影响。方法建立C57BL/6J小鼠Lewis肺癌模型30只,脾虚Lewis肺癌模型90只,分为4组,Lewis组(对照组)、脾虚Lewis组(模型组)、益气除痰方低剂量组、高剂量组,每组30只,对照组、模型组予生理盐水0.5mL、治疗组予益气除痰方1.0及3.0g/kg处理,每天1次,灌胃21天后,每组各处死10只检测肿瘤体积、重量、肺转移灶,并用免疫组化法比较各组肿瘤组织PRDX-1,PRDX-6的表达,其余荷瘤鼠观察生存期。结果益气除痰方高剂量组Lewis肺癌小鼠肿瘤体积、瘤重、肺转移灶明显下降,分别为(1.14±0.30)cm3、(0.83±0.26)g、(6.20±2.53)个,而对照组分别为(2.83±0.35)cm3、(2.08±0.28)g、(8.60±1.84)个,模型组分别为(2.29±0.49)cm3、(1.67±0.33)g、(8.70±2.00)个;高剂量组小鼠中位生存期为(29.00±0.89)天,平均生存时间为(29.60±0.53)天,与对照组(22.00±0.75)天、(22.90±0.50)天,模型组(21.00±0.53)天、(20.95±0.44)天比较生存期明显延长;高剂量组免疫组化肿瘤组织PRDX-1、PRDX-6表达量明显下降,分别为0.15±0.03、0.12±0.02,对照组分别为0.52±0.07、0.43±0.06,模型组分别为0.61±0.09、0.56±0.07。高剂量组与对照组、模型组比较差异有统计学意义(P<0.05,P<0.01)。高剂量组抑瘤率为50.30%,生命延长率为41.29%,优于低剂量组(P<0.05)。结论益气除痰方能抑制脾虚小鼠Lewis肺癌的生长,延长生存期,其机制可能与降低PRDX-1,PRDX-6的过量表达有一定关系。

英文摘要:

Objective To investigate the effects of Yiqi Chutan Recipe (YCR,a Chinese herbal prescription for invigorating qi and removing phlegm) on the growth and metastasis of tumor,survival time,and the expressions of peroxiredoxin (PRDX-1 and PRDX-6) in tumor tissue of C57BL/6J mice bearing Lewis lung carcinoma. Methods Lewis lung carcinoma cells were transplanted to 90 C57BL/6J mice receiving preconditioning for inducing Pi-deficiency syndrome and divided into three groups treated respectively with saline,high dose YCR (3.0 g/kg) and low dose YCR (1.0 g/kg) once a day via gastric infusion. Besides,a group of 30 healthy mice simply received tumor cell transplantation was set up for controls. Ten mice selected from each group were sacrificed 21 days later,the size,weight and lung metastasis foci of tumor in mice were measured,and expressions of PRDX-1 and PRDX-6 in tumor tissue were detected using immunohistochemical method. The survival time of the remained 20 mice in each groups was observed. Results Tumor size,weight and the numbers of lung metastatic foci were (1.14±0.30)cm3,(0.83±0.26)g,(6.20±2.53)foci in the high dose YCR treated group,which were significantly lower than those in the control group [(2.83±0.35)cm3,(2.08±0.28)g,and (8.60±1.84)foci] respectively,also lower than those in the saline treated group [(2.29±0.49)cm3,(1.67±0.33)g and (8.70±2.00) foci]. The median survival time in the three groups,in above order,were 29.00±0.89 days,22.00±0.75 days and 21.00±0.53 days; the average survival time in them 29.60±0.53 days,22.90±0.50 days and 20.95±0.44 days; the PRDX-1 expression were 0.15±0.03,0.52±0.07 and 0.61±0.09; and the PRDX-6 expression were 0.12±0.02,0.43±0.06 and 0.56±0.07,all showed significant difference in comparing the indices in the high dose treated group with those in the control group and in the saline treated group (P<0.05 or P<0.01). The tumor growth inhibition rate was 50.30% in the high dose YCR group with life prolongation rate of 41.29%,all better than those in the low dose YCR treated group (P<0.05). ConclusionsYCR can remarkably inhibit the growth and metastasis of Lewis lung carcinoma in mice with Pi-asthenia syndrome,prolong their survival period,and its mechanism is possibly related to the reduction of over expressed PRDX-1 and PRDX-6.

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