人参皂苷Rb<sub>1</sub>对氧化型低密度脂蛋白诱导的人单核细胞源树突状细胞免疫成熟的影响

作者	单位
史大卓	中国中医科学院西苑医院
马晓娟	中国中医科学院西苑医院
刘红樱	中国中医科学院西苑医院上海复旦大学附属中山医院
葛均波	上海复旦大学附属中山医院

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中文摘要:

目的研究中药单体人参皂苷Rb1(ginsenoside Rb1,GRb1)对氧化型低密度脂蛋白(oxidized low density lipoprotein,OX-LDL)诱导的人单核细胞源树突状细胞(dendritic cells,DCs)免疫成熟的影响。方法采用免疫磁珠法分离人外周血CD14+单核细胞,诱导分化为未成熟DCs,随机分为磷酸盐缓冲液(PBS)组、GRb1组、环格列酮组、OX-LDL组、GRb1预处理加OX-LDL组、环格列酮预处理加OX-LDL组。流式细胞仪检测DCs表型(CD40、CD1a和HLA-DR)和吞噬功能变化,酶联免疫吸附法检测细胞培养上清细胞因子白细胞介素12(IL-12)、肿瘤坏死因子-α(TNF-α)浓度变化。结果与OX-LDL组比较,GRb1预处理加OX-LDL组DCs表面分子CD40(67.40±1.62vs.145.69±14.86)、CD1a(79.64±3.04vs.159.89±6.09)和HLA-DR(46.43±2.85vs.99.33±17.11)的表达显著降低(P<0.01),DCs的吞噬功能明显提高[(88.13±1.06)%vs.(25.90±5.77)%,P<0.01],细胞培养上清液IL-12[(88.65±5.59)ng/Lvs.(716.69±36.35)ng/L]和TNF-α[(133.27±11.98)ng/Lvs.(968.10±36.42)ng/L]水平明显下降(P<0.01)。与OX-LDL组比较,环格列酮预处理加OX-LDL组DCs表面分子表达和炎症细胞因子分泌也明显降低(P<0.01),DCs吞噬功能也明显增强,但与GRb1预处理组比较无明显性差异(P>0.05)。结论 GRb1可明显抑制OX-LDL诱导的DCs的免疫成熟,与西药环格列酮具有相似的功效。

英文摘要:

Objective To examine the effect of ginsenoside Rb1(GRb1) on the immune maturation of monocyte-derived dendritic cells(DCs) induced by oxidized low-density lipoprotein(OX-LDL).Methods Human monocytes purified by CD14+ immuno-magnetic beads were differentiated and induced into immature DCs,which were randomly divided into 6 groups,Group A treated with PBS,Group B treated with OX-LDL,Group C and D treated respectively with GRb1 and ciglitazone,Group E and F were pretreated with the two testing drugs respectively followed by OX-LDL.The immuno-phenotypic expression(CD40,CD1a,and HLA-DR) and endocytosis function of DCs were examined using flow cytometry,the concentration of interleukin-12(IL-12) and tumor necrosis factor α(TNF-α) in the culture supernatants were measured with ELISA.Results Compared with Group B,Group E showed significantly lowered immuno-phenotypic expression of DCs in terms of CD40(67.4±1.62 vs.145.69±14.86),CD1a(79.64±3.04 vs.159.89±6.09),and HLA-DR(46.43±2.85 vs.99.33±17.11),as well as higher endocytosis level(88.13%±1.06% vs.25.90%±5.77%,all P<0.01).Meantime,the serum levels of IL-12(88.65±5.59 ng/L vs.716.69±36.35 ng/L) and TNF-α(133.27±11.98 ng/L vs.968.10±36.42 ng/L) obviously decreased(P<0.01).The surface molecular expression of DCs and the secretion of inflammatory factors in Group F also obviously decreased,showing insignificant difference from Group E(P>0.05).Conclusion GRb1 could obviously inhibit the OX-LDL-induced maturation of DCs,showing similar effects to ciglitazone.

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