姜黄素对快速老化小鼠学习与记忆功能及海马Ca<sup>2+</sup>/CaMKⅡ水平的影响

作者	单位
周鹏	温州医学院解剖学教研室
唐茂林	温州医学院解剖学教研室
孙淑红	温州医学院解剖学教研室
孙臣友	温州医学院解剖学教研室
戚双双	温州医学院第二附属医院药剂科
崔怀瑞	温州医学院解剖学教研室

摘要点击次数: 2004

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中文摘要:

目的探讨不同浓度的姜黄素对快速老化小鼠(senescence-accelerated mouse,SAM)学习与记忆的影响和可能的机制。方法将小鼠随机分为SAMR1正常对照组,SAMP8模型对照组,SAMP8+溶剂(花生油)对照组以及SAMP8+低、中、高浓度姜黄素处理组,灌胃持续25天。实验结束次日,通过Morris水迷宫测试各组小鼠学习和记忆成绩的变化;测定各组小鼠海马组织[Ca2+]i浓度;通过Western blot和RT-PCR观测Ca2+/钙调蛋白依赖性蛋白激酶Ⅱ(calmodulin-dependent protein kinaseⅡ,CaMKⅡ)和钙调蛋白(calmodulin,CaM)mR-NA在海马组织的表达情况。结果 SAMP8模型对照组小鼠隐蔽平台的逃避潜伏期明显延长;海马组织[Ca2+]i明显增高;海马膜中CaMKⅡ表达量降低;海马组织CaMmRNA的水平明显降低(P<0.05,P<0.01)。经中、高浓度姜黄素处理的SAMP8小鼠隐蔽平台的逃避潜伏期显著缩短(P<0.01)。经低、中和高浓度姜黄素处理的SAMP8组小鼠海马组织[Ca2+]i均明显降低;海马组织膜中CaMKⅡ表达量均明显增加;海马组织CaMmRNA水平均明显增高(P<0.05,P<0.01)。结论姜黄素能够通过降低海马组织[Ca2+]i超载,增加海马组织CaMmRNA水平及海马膜上CaMKⅡ表达来改善SAMP8小鼠的学习和记忆能力,此作用呈剂量依赖效应。

英文摘要:

Objective To explore effect of curcumin in different concentrations on learning and memory of senescence-accelerated mice(SAM) and their possible mechanisms.Methods Mice were randomly divided into six groups:the SAMR1 normal control group,the SAMP8 model control group,the SAMP8+solvent(the peanut oil) control group,SAMP8+ low,middle and high dose curcumin groups.Mice were gastrogavaged for 25 successive days.On the next day of ending the experiment,changes of learning and memory in mice of each group were observed by Morris water maze.The hippocampal [Ca2+]i was determined.Expressions of hippocampal calmodulin-dependent protein kinase Ⅱ(CaMKⅡ) and Calmodulin(CaM) mRNA were detected using Western blot and reverse transcription polymerase chain reaction(RT-PCR) respectively.Results The latency to find the hidden platform was remarkably prolonged,the hippocampal [Ca2+]i was markedly increased,the expression of CaMKⅡ in the hippocampal membrane and the level of hippocampal CaM mRNA were significantly reduced in the SAMP8-model control group(P<0.05,P<0.01).The latency to find the hidden platform was remarkably shortened in the SAMP8+ middle dose curcumin and the SAMP8+high dose curcumin groups(P<0.01).The hippocampal [Ca2+]i was markedly lowered,the expression of CaMKⅡ in the hippocampal membrane and the level of hippocampal CaM mRNA obviously increased in the SAMP8+ low,middle and high dose curcumin groups(P<0.05,P<0.01).Conclusion Curcumin could improve learning and memory Ca2+/capacities of SAM by lowering hippocampal [Ca2+]i overload,increase the hippocampal CaM mRNA level and CaMKⅡ expression in the hippocampal dose-dependently.

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