区分NZB/WF1狼疮鼠不同体质的实验研究

作者	单位
姚重华	上海中医药大学附属龙华医院风湿科
杨亚馗	上海中医药大学附属龙华医院风湿科
薛轶燕	上海中医药大学附属龙华医院风湿科
王骁	上海中医药大学附属龙华医院风湿科
田雨	上海中医药大学附属龙华医院风湿科
覃光辉	上海中医药大学附属龙华医院风湿科
苏励	上海中医药大学附属龙华医院风湿科
曲环汝	上海中医药大学附属龙华医院风湿科

摘要点击次数: 2104

全文下载次数: 0

中文摘要:

目的检测划分NZB/WF1狼疮鼠体质,将其分为寒、常、热3种体质,并证实这些体质差异的客观存在。方法采用上海中医药大学实验中医学教研室方肇勤教授课题组建立的"实验小鼠四诊工作站",检测158只NZB/WF1小鼠体重、腋温、心率、小鼠35s活动度、爪尾色泽(r值)等指标,根据公式:权重总值=爪校正值×1+尾校正值×0.2+腋温校正值×0.7+心率校正值×0.05+跨格数×0.025计算总权重,根据"总权重值高,体质越热;总权重值低,体质越寒"的原则将NZB/WF1小鼠划分为寒体、常体和热体3种体质小鼠。分别检测不同体质小鼠肾组织羟脯氨酸含量、结缔组织生长因子(connective tissue growth factor,CTGF)、转化生长因子β1(transforming growth factor-β1,TGF-β1)基因表达差异和肾组织免疫荧光染色情况。结果在158只NZB/WF1小鼠中,共分出热体小鼠33只,寒体小鼠34只,常体小鼠91只。常体和寒体小鼠的肾组织羟脯氨酸含量高于热体组(P<0.01),而常体和寒体间比较,差异无统计学意义。寒体及热体小鼠CTGF基因表达量显著高于常体小鼠(P<0.05),寒体与热体间比较,差异无统计学意义。寒体小鼠TGF-β1基因表达量低于常体及热体小鼠,但差异无统计学意义。不同体质小鼠肾组织免疫荧光染色也存在一定的差异。结论 NZB/WF1狼疮鼠存在体质差别。

英文摘要:

Objective To classify NZB/W F1 lupus mice into three different constitutions,i.e.the cold,normal,and hot constitutions,and to prove the objective existence of the difference among them.Methods Using the Four Diagnosis Work Station for Mice(founded by Prof.FANG Zhao-qin,the Research Faculty of Experimental Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine),the body weight,the armpit temperature,the heart rate,the 35-s activities,and the color of their tails and claws(r value) were detected.The total weight was calculated according to the formula:The total weight sum=the correction value of claws×1+the correction value of tail×0.2 + the correction value of the armpit temperature×0.7 + the correction value of heart rate×0.05 + the number of the quadrant crossing×0.025.The NZB/W F1 lupus mice were classified into the three different constitutions according to the higher total weight sum,the hotter the constitution,the lower total weight sum,the colder the constitution.The hydroxyproline content,connective tissue growth factor(CTGF),and transforming growth factor-β1(TGF-β1) gene expression difference in the renal tissue were detected and the immunofluorescence staining observed.Results Among the 158 NZB/W F1 lupus mice,33 mice were classified into the hot constitution,34 into the cold constitution,and 91 into the normal constitution.The renal hydroxyproline content in mice of normal and cold constitutions were higher than that of mice of the hot constitution(P<0.01).No statistical difference was shown between mice of the normal constitution and mice of the cold constitution.The CTGF gene expression level was significantly higher in mice of the cold constitution and mice of the hot constitution than in mice of the normal constitution(P<0.05).No significant difference was found between mice of the cold constitution and mice of the hot constitution.Lower level of TGF-β1 expression existed in mice of the cold constitution than in mice of the normal constitution or mice of the hot constitution,showing insignificant difference.The immunofluorescence stain of the renal tissue among the three constitutions also showed some difference.Conclusion Constitution difference did exist among NZB/W F1 lupus mice.

关闭