四慧合剂对疲劳模型大鼠学习记忆及海马NMDA受体亚基NR2A、NR2B及EphB2受体表达的影响

Effect of Sihui Mixture on the Learning and Memory Ability,mRNA Expressions of Hippocampal NMDA Subunit NR2A and NR2B,and EphB2 Receptor in Fatigue Rats

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中文关键词: 突触可塑性 N-甲基-D-天冬氨酸受体2A亚基 N-甲基-D-天冬氨酸受体2B亚基酪氨酸激酶家族受体B2 中枢性疲劳学习记忆

英文关键词:the synaptic plasticity N-methyl D-aspartate receptor 2A subunit N-methyl D-aspartate receptor 2B subunit Eph receptor B2 central nervous system fatigue learning and memory

基金项目:国家自然科学基金资助项目(No.30873217)

作者	单位
许展嘉	北京中医药大学基础医学院
谢华	北京中医药大学基础医学院
王霁	北京中医药大学基础医学院
宋月晗	北京中医药大学基础医学院
马佳美	北京中医药大学基础医学院
刘燕	北京中医药大学基础医学院
刘晓兰	北京中医药大学基础医学院
李峰	北京中医药大学基础医学院
范新六	北京中医药大学基础医学院

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中文摘要:

目的研究疲劳模型大鼠学习记忆及海马N-甲基-D-天冬氨酸(N-methyl D-aspartate,NMDA)受体NR2A、NR2B亚基及EphB2受体表达的变化,并观察四逆散、生慧汤、四慧合剂对其影响。方法采用多平台法,进行连续睡眠剥夺168h复制中枢疲劳模型,将实验动物随机分为正常对照组、模型组、四逆散组、生慧汤组、四慧合剂组,每组10只;分别在睡眠剥夺6、30、54、78、102、126、150h时间点灌胃给药及蒸馏水。应用Y迷宫观察大鼠学习记忆能力的变化,并使用实时荧光定量PCR(Real-time PCR)技术定量分析大鼠海马NR2A、NR2B亚基及EphB2受体mRNA表达的变化。结果与正常对照组比较,模型组大鼠Y迷宫正确百分率明显下降(P<0.05),海马NR2B和EphB2的mRNA表达明显减少(P<0.01),NR2A表达无明显变化。与模型组比较,四慧合剂组能明显提高大鼠迷宫成绩和海马NR2A、EphB2的mRNA表达(P<0.01),而四逆散组、生慧汤组差异无统计学意义。与四逆散组比较,四慧合剂组EphB2的mRNA表达明显增多(P<0.01)。生慧汤组较模型组亦能明显增加NR2A的mRNA表达(P<0.01)。结论中枢疲劳能导致大鼠迷宫成绩下降和海马NR2B和EphB2基因表达下调。四慧合剂能增加大鼠的学习记忆能力,可能是通过上调大鼠海马NR2B和EphB2的mRNA表达所致。

英文摘要:

Objective To study the learning and memory ability,expressions of hippocampal N-methyl D-aspartate(NMDA) subunit NR2A and NR2B,and EphB2 receptor in fatigue rats,and to observe effects of Sini Powder,Shenghui Decoction,and Sihui Mixture on them.Methods The central nervous system fatigue model was duplicated by paradoxical sleep deprivation for 168 h using multiple platform method.Experimental rats were randomly divided into the normal control group,the model group,the Sini Powder group,and the Shenghui Decoction group,ten in each.Corresponding medicines and distilled water were given to them by gastrogavage at 6,30,54,78,102,126,and 150 h after sleep deprivation.Changes of the learning and memory ability were observed using Y maze.mRNA expressions of NMDA subunit NR2A and NR2B,and EphB2 receptor in fatigue rats were quantitatively analyzed using Real-time PCR.Results Compared with the normal control group,the Y maze correct percentage in the model group obviously decreased(P<0.05),mRNA expressions of NR2B and EphB2 obviously decreased(P<0.01),with no obvious change in NR2A.Compared with the model group,Sihui Mixture could obviously improve Y maze results and mRNA expressions of NR2A and NR2B,and EphB2(P<0.01).No statistical difference was found between the Sini Powder group and the Shenghui Decoction group.Compared with the Sini Powder group,mRNA expressions of EphB2 obviously increased in the Sihui Mixture group(P<0.01).mRNA expression of NR2A could be more obviously increased in the Shenghui Decoction group than in the model group(P<0.01).Conclusion The central nervous system fatigue could result in decreased Y maze results and gene expressions of hippocampal NR2B and EphB2.Sihui Mixture could improve rats’ learning and memory ability,which might be possibly achieved through up-regulating mRNA expressions of hippocampal EphB2 and NR2B.

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