消痰散结方对人胃癌裸鼠移植瘤血管生成拟态的影响

作者	单位
周伟	第二军医大学长征医院中医科
魏品康	第二军医大学长征医院中医科
李勇进	第二军医大学长征医院中医科

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中文摘要:

目的探讨消痰散结方对人胃癌裸鼠移植瘤血管生成拟态(vasculogenic mimicry,VM)的影响及其机制。方法 30只裸鼠右前肢腋部皮下移植人胃癌瘤块建立荷瘤模型。造模后的荷瘤鼠随机分为模型组(10只)、消痰散结组(10只)和多西环素组(10只),分别给予相应药物灌胃治疗4周后,脱颈椎处死荷瘤鼠。剥取肿瘤,称取瘤质量,计算抑瘤率;计数肿瘤中VM的数量;运用免疫组织化学法检测肿瘤中基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)和基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)蛋白的表达。结果消痰散结组和多西环素组裸鼠移植瘤质量明显低于模型组(P<0.01)。消痰散结组和多西环素组VM个数分别为(24.50±3.03)和(14.70±1.34),与模型组(33.10±2.64)比较,差异有统计学意义(P<0.01)。消痰散结组和多西环素组MMP-2和MMP-9蛋白的阳性表达低于模型组,差异均有统计学意义(P<0.01)。结论消痰散结方可抑制裸鼠移植瘤VM的形成,其机制可能与下调MMP-2和MMP-9蛋白表达有关。

英文摘要:

Objective To study the effect of Xiaotan Sanjie Recipe(XTSJD) and its mechanism on vasculogenic mimicry(VM) of human gastric cancer xenografts in nude mice.Methods The tumor-bearing mice model was established by subcutaneous inoculating with xenografts of human gastric cancer into the right armpit of 30 BALB/c nude mice.After modeling,the tumor-bearing mice were randomly divided into the normal saline group,the XTSJD group,and the doxycycline hyclate(DH) group,10 in each.And the mice were administered with corresponding medicine by gastrogavage for 4 weeks.Then all mice were killed by cervical dislocation.The tumor mass were weighed and the tumor inhibition rate calculated.The amount of VM in tumor was counted.Expressions of matrix metalloproteinase(MMP)-2 and MMP-9 were tested using immunohistochemical method.Results Tumor weight in the XTSJD group and the DH group decreased significantly when compared with the NS group(P<0.01).The amount of VM in the XTSJD group(24.50±3.03) and the DH group(14.70±1.34) was significantly less than that in the NS group(33.10±2.64)(P<0.01).The positive expressions of MMP-2 and MMP-9 in the XTSJD group and the DH group was significantly lower than that in the NS group(P<0.01).Conclusion XTSJD could inhibit the formation of VM in xenografted tumor of nude mice.The mechanism might be correlated with the down-regulation of MMP-2 and MMP-9 expressions.

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