丹酚酸B对实验性肝纤维化大鼠肝组织CD14表达的影响

作者	单位
杨胜兰	华中科技大学同济医学院附属协和医院中西医结合科
刘建国	华中科技大学同济医学院附属协和医院中西医结合科
黄芳	湖北中医药大学中药教研室
丁艳蕊	湖北中医药大学中药教研室

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中文摘要:

目的观察丹参提取物丹酚酸B对四氯化碳(CCl4)致实验性肝纤维化大鼠肝组织表达白细胞分化抗原14(CD14)基因和蛋白的影响。方法将30只SD大鼠随机分为3组:CCl4模型组(简称模型组)、丹酚酸B治疗组(简称治疗组)及空白对照组(简称对照组)。比较各组大鼠肝脏病理变化的同时采用自动生化分析仪检测肝功能,基质染色法测定血清内毒素含量,放射免疫法测定血浆肿瘤坏死因子-α(TNF-α)的含量,以RT-PCR和免疫组化法检测大鼠肝组织CD14 mRNA和蛋白的表达。结果 CCl4诱导的肝纤维化模型大鼠肝功能、肝纤维化程度、血清内毒素水平及血浆TNF-α含量及CD14 mRNA和蛋白的表达较对照组显著增加(P<0.01);治疗组与模型组比较,上述指标均降低(P<0.01)。结论丹酚酸B能拮抗CCl4诱导的大鼠肝纤维化,其作用机制可能与其能下调肝组织CD14的表达、阻滞内毒素信号转导通路有关。

英文摘要:

Objective To observe the effect of salvianolic acid B(SAB),an extract from Radix Salviae miltiorrhizae,on expression of leucocyte differentiation antigen 14(CD14) in the liver tissue of experimental rats with carbon tetrachloride(CCl4)-induced liver fibrosis.Methods Thirty SD rats were randomly divided into three groups,the model group,the treated group,and the control group.The pathological fibrosis changes in liver of rats were observed.Meantime,their liver function was detected by automatic biochemical analyzer.Serum content of endotoxin was assayed by matrix staining,and plasma content of tumor necrosis factor-α(TNF-α) was detected by radioimmunoassay.mRNA and protein expressions of CD14 in the liver tissue were measured using reverse transcriptional-polymerase chain reaction and immunohistochemistry respectively.Results All the laboratory parameters,including liver function,degree of liver fibrosis,serum endotoxin levels,plasma TNF-α contents,and CD14 mRNA and protein expressions in the model group were higher than those in the control group(all P<0.01).All the aforesaid indices were lowered more in the treated group than in the model group(all P<0.01).Conclusions SAB could antagonize the CCl4 induced liver fibrosis in rats.Its mechanism of action was possibly correlated with its effects on down-regulating hepatic CD14 expression and blocking the endotoxin signal transduction pathway.

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