大黄素联合吉西他滨对体外人胰腺癌细胞株BxPC-3生长及凋亡的影响

作者	单位
曾勇	温州医学院附属第二医院
童洪飞	温州医学院附属第二医院
邱麦轩	温州医学院附属第二医院
刘岸	温州医学院附属第二医院
林胜璋	温州医学院附属第二医院

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中文摘要:

目的探讨大黄素(emodin)联合吉西他滨(gemcitabine)对人胰腺癌细胞株BxPC-3的影响。方法人胰腺癌细胞株BxPC-3经不同浓度大黄素(0、10、20、40、80、160μmol/L)分别作用24、48、72h;及大黄素(40μmol/L)联合吉西他滨(20μmol/L)作用72h。应用Cell Counting Kit-8(CCK-8)法检测大黄素及大黄素联合吉西他滨对BxPC-3细胞的增殖抑制作用;流式细胞术检测BxPC-3细胞凋亡情况。结果大黄素对BxPC-3细胞的增殖抑制作用呈明显浓度和时间依赖性,40μmol/L大黄素作用于BxPC-3细胞24、48、72h后的细胞存活率分别为79.39%、46.35%、45.44%;大黄素(40μmol/L)联合协同吉西他滨(20μmol/L)作用72h后胰腺癌BxPC-3细胞的存活率仅为26.62%,与吉西他滨组(42.78%)及大黄素组(47.18%)比较,差异有统计学意义(P<0.05)。大黄素(40μmol/L)和吉西他滨(20μmol/L)单独作用于BxPC-3细胞24h后,诱导胰腺癌细胞早期凋亡率分别为(4.70±1.54)%和(11.20±1.41)%,与联合组[细胞凋亡率为(20.60±3.23)%]比较,差异均有统计学意义(P<0.05)。结论大黄素可显著抑制人胰腺癌BxPC-3细胞生长;大黄素有效增强吉西他滨对人胰腺癌BxPC-3细胞的增殖抑制作用;其协同作用以诱导胰腺癌细胞凋亡方式为主。

英文摘要:

Objective To explore the effect of emodin combined gemcitabine(E&G) on human pancreatic cancer cell line BxPC-3 in vitro.Methods BxPC-3 cells were treated with emodin alone in different concentrations(0,10,20,40,80,and 160 μmol/L,respectively) for 24,48,and 72 h,and E&G(emodin 40 μmol/L+ gemcitabine 20 μmol/L) for 72 h.The inhibition on BxPC-3 cell proliferation was detected by Cell Counting Kit-8 assay and the cell apoptosis of BxPC-3 was determined using flow cytometry.Results Emodin obviously suppressed the proliferation of BxPC-3 cells in a dose-and time-dependent manner.The survival rates of BxPC-3 cells by 40 μmol/L emodin for 24,48,and 72 h were 79.39%,46.35%,and 45.44%,respectively,while the survival rate of BxPC-3 cells acted by 72-h E&G was only 26.62%,showing significant difference from that by gemecitabine alone(42.78%) and the emodin alone(47.18%).The early apoptotic ratio of BxPC-3 cells induced by 24 h emodin(40 μmol/L) and gemecitabine(20 μmol/L) were 4.70%±1.54% and 11.20%±1.41% respectively,while early apoptotic ratio of BxPC-3 cells induced by E&G was 20.60%±3.23%,showing significant difference from that induced by emodin or gemecitabine alone(P<0.05).Conclusions Emodin could significantly inhibit BxPC-3 cell growth.It could act synergistically with gemcitabine to inhibit the tumor proliferation of BxPC-3 cells.Its syndergistic action was achieved mainly through inducing pancreatic cancer cell apoptosis.

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