| 林传权,李茹柳,郭文峰,高小玲,陈玉龙,陈蔚文.溃疡性结肠炎脾胃虚实证候核糖体蛋白基因表达研究[J].中国中西医结合杂志,2011,31(5):603-607 |
| 溃疡性结肠炎脾胃虚实证候核糖体蛋白基因表达研究 |
| Study on Ribosomal Protein Gene Expression in Patients with Ulcerative Colitis of Pi-asthenic Syndrome and Pi-sthenic Syndrome |
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| DOI： |
| 中文关键词: 脾虚证 湿热证 基因芯片 核糖体蛋白 溃疡性结肠炎 |
| 英文关键词:Pi-asthenic syndrome dampnese-heat syndrome gene array ribosomal protein ulcerative colitis |
| 基金项目:国家自然科学基金重大研究计划重点项目(No.90209004);广东省自然科学基金重点项目(No.05102323);上海市教育委员会E-研究院建设计划项目(No.E03008) |
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| 中文摘要: |
| 目的研究健康人、溃疡性结肠炎(ulcerative colitis,UC)脾虚证和湿热证患者核糖体蛋白(ribo-somal protein,RP)基因表达差异情况,为从蛋白合成角度理解"脾为气血生化之源"提供实验依据。方法制作RP基因芯片,对4名健康人、4例UC脾虚证和4例UC湿热证患者结肠黏膜进行检测,应用BRB-TOOL(3.9)软件包进行数据分析,对差异基因进行生物信息学分析。结果成功制作了包括77条RP基因,2条RP类似基因(RPL26-like1、RPL7-like1)的低密度芯片。UC(脾虚证+湿热证)患者与健康人之间有12条差异基因,全部为下调基因;湿热证与健康人之间有19条差异基因,全部呈下调趋势;脾虚证与健康人有3条差异表达基因,全部为下调基因;脾虚证与湿热证有6条差异表达基因,全部为上调基因。聚类分析显示健康人与UC患者样品可以根据本芯片的基因表达谱进行分类,湿热证和脾虚证可以通过聚类分开。多个差异基因有共同转录调节因子。结论成功制作了RP基因芯片,UC脾虚证和湿热证RP基因表达下调;健康人、UC脾虚证和UC湿热证都有各自相应的RP基因表达谱。 |
| 英文摘要: |
| Objective To study differential expression profiles of ribosomal protein (RP) genes in healthy subjects and ulcerative colitis (UC) patients of Pi-asthenic syndrome (PAS) and of dampness-heat syndrome (DHS),thus providing experimental bases for "Pi as the source of qi and blood" theory from the view of protein synthesis. Methods RP genes arrays were made. The mucous membrane of colon was detected in four UC patients of PAS (UC-PAS),four UC patients of DHS (UC-DHS),and four healthy subjects (N),and data analyzed using BRB-TOOL Software Package (3.9). Bioinformatics analyses were conducted in differential genes. ResultsLow-density RP gene chips were successfully produced,including 77 RP genes and two RP like genes (RPL26-like1 and RPL7-like1). There were twelve differential genes between UC (PAS+DHS) and N,all of which were down-regulated genes. There were nineteen differential genes between UC-DHS and N,all of which showed down-regulating tendency. There were three differential genes between UC-PAS and N,all of which were down-regulated genes. There were six differential genes between UC-PAS and UC-DHS,all of which were up-regulated genes. Cluster analysis showed that normal and UC samples of this chip can be classified according to gene expression profiles,and UC-PAS and UC-DHS can be classified by clustering. Various differential genes had a common transcription regulatory factor. Conclusions RP genes arrays were successfully produced. RP gene expressions were down-regulated in UC-PAS and UC-DHS. Corresponding gene expression profiles were shown in N,UC-PAS and UC-DHS. |
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