表没食子儿茶素没食子酸酯对乳胞素诱导PC12细胞损伤的影响

作者	单位
竺飞燕	温州医学院附属东阳医院
赵丹	温州医学院附属东阳医院
张雄	温州医学院附属东阳医院
王小同	温州医学院附属东阳医院
唐兰英	温州医学院附属东阳医院
胡丹	温州医学院附属东阳医院
戴美芬	温州医学院附属第二医院神经内科
陈瑛	温州医学院附属东阳医院

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中文摘要:

目的探讨表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)对乳胞素(lactacystin)诱导的PC12细胞损伤的影响。方法接种PC12细胞培养24h后分别给予干预。实验分为5组:正常对照组,10μmol/L乳胞素损伤组,不同浓度EGCG预处理组(终浓度5、10、50μmol/L)。分别用MTT比色法检测细胞活性,Hoechst33258染色观察凋亡时细胞核形态的变化,流式细胞仪(FCM)检测细胞凋亡率。结果 5、10、50μmol/L EGCG对乳胞素诱导的PC12细胞损伤具有一定的保护作用。与乳胞素损伤组[(61.22±1.02)%]比较,5、10、50μmol/L EGCG预处理组细胞活力明显上升,分别为(66.99±1.30)%、(66.67±0.65)%、(73.40±0.67)%。Hoechst33258染色发现乳胞素损伤组较多胞核出现固缩凝集,而EGCG预处理组则较少。FCM检测也提示EGCG预处理可降低细胞的凋亡率,正常对照组、乳胞素损伤组、EGCG预处理组(5、10、50μmol/L)分别为3.0%、60.4%、59.8%、57.5%和38.6%。结论 EGCG可减轻乳胞素诱导的PC12细胞损伤。

英文摘要:

Objective To study the effect of epigallocatechin gallate (EGCG) against lactacystin induced PC12 cell injury. Methods The inoculated rat PC12 cells were cultured for 24 h,followed by intervention. The cells were divided into 5 groups,i.e.,the normal control group,10 μmol/L lactacystin injury group,and the EGCG pretreated groups (at the final concentration of 5,10,and 50 μmol/L,respectively). The cytoactivity was detected by MTT colorimetry. Morphological changes of the cell nucleus were observed by Hoechst 33 258 staining,and the apoptosis ratio was detected by flow cytometry (FCM). Results EGCG at different doses showed protective effect on lactacystin-induced PC12 cell injury. Compared with the lactacystin injury group [(61.22±1.02)%],the cytoactivity in EGCG pretreated groups at the final concentration of 5,10,and 50 μmol/L,respectively increased obviously to (66. 99±1.30)%,(66.67±0.65)%,and (73.4±0.67)%,respectively. Hoechst 33 258 staining found that more nuclear pyknosis and aggregation occurred in the lactacystin injury group,but less occurred in EGCG pretreated groups. FCM indicated that the apoptosis ratio was reduced by EGCG pretreatment. It was 3.0%,60.4%,59.8%,57.5%,and 38.6%,respectively in the normal control group,the lactacystin injury group,and EGCG pretreated groups (at the final concentration of 5,10,and 50 μmol/L,respectively). Conclusion EGCG could attenuate lactacystin induced PC 12 cell injury.

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