黄芩苷抑制beta淀粉样蛋白诱导的海马COX-2蛋白表达

作者	单位
晏勇	重庆医科大学附属第一医院神经内科
晏宁	重庆医科大学附属第二医院神经内科
马勋泰	重庆医科大学附属第一医院神经内科
李振华	重庆医科大学附属第一医院神经内科
蒋青松	重庆医科大学基础医学院药学院
韩宇	重庆医科大学附属第一医院神经内科
承欧梅	重庆医科大学附属第一医院神经内科

摘要点击次数: 1267

全文下载次数: 1271

中文摘要:

目的探讨中药单体黄芩苷对阿尔茨海默病(Alzheimer′s disease,AD)大鼠的脑保护作用及其可能的作用机制。方法将36只健康雄性Wistar大鼠随机分成对照组、AD组、黄芩苷组,每组12只。AD组及黄芩苷组采用双侧海马内注射beta淀粉样蛋白(Aβ1-40),建立AD大鼠模型。对照组采用相同方法行假手术,双侧海马内仅注射等量的0.9%氯化钠溶液。黄芩苷组术前、术后连续腹腔注射黄芩苷[40mg/(kg.d)],每天1次,连续7天。AD组和对照组也经相同时间相同步骤给予相同体积的缓冲液腹腔注射。采用免疫印迹法测定海马环氧合酶-2(COX-2)的表达,T迷宫实验观察大鼠的空间学习记忆能力,HE染色观察神经元组织学改变。结果 T迷宫实验结果显示:AD组[(28.33±7.50)%]和黄芩苷组[(38.33±7.50)%]大鼠自发交替选择率减小,与对照组[(61.67±7.50)%]比较,差异均有统计学意义(P<0.05),两组间比较差异有统计学意义(P<0.05)。HE染色结果显示:与黄芩苷组比较,AD组皮层、海马神经元变性坏死改变更明显。免疫印迹法分析显示:AD组COX-2表达明显增高,黄芩苷组COX-2表达明显降低(P<0.05)。结论黄芩苷能减轻beta淀粉样蛋白所致的脑损伤,可能与抑制COX-2的表达有关。

英文摘要:

Objective To study the brain protection of baicalin on rats with Alzheimer′s disease (AD) and its probable mechanism of action. Methods Thirty-six male healthy Wistar rats were randomly divided into the sham-operative group,the AD group,and the baicalin group,twelve in each. β-amyloid protein 1-40 was injected to the bilateral hippocampus of rats in the AD group and the baicalin group to establish the AD rat model. The sham operation was performed to rats of the sham-operative group in the same way. Equal volume of 0.9% sodium chloride solution was injected to the bilateral hippocampus of rats in the sham-operative group. Baicalin was intraperitoneally injected at the daily dose of 40 mg/kg to rats in the baicalin group before and after operation,once daily for 7 successive days. Equal volume of buffer solution was intraperitoneally injected to rats in the sham-operative group and the AD group in the same procedures at the same time points. The expression of hippocampal cyclooxygenase-2 (COX-2) was determined by Western blot. The spacial learning memory capacities was observed using T-morris test. Histological changes were observed using hematoxylin-eosin (HE) staining. Results Results of the T-morris test showed the spontaneous alternation selective ratio decreased in the AD group (28.33%±7.50%) and the baicalin group (38.33%±7.50%) (both P<0.05) when compared with the sham-operative group (61.67%±7.50%). There was significant difference between the AD group and the baicalin group (P<0.05). Results of HE staining showed degeneration and necrosis of cortical and hippocampal neurons in the AD group and the baicalin group. Changes in the AD group were more obvious. Results of Western blot showed the expression of hippocampal cyclooxygenase (COX-2) obviously increased in the AD group,while it obviously decreased in the baicalin group (P<0.05). Conclusion Baicalin could alleviate beta-amyloid protein induced brain injury,which might be associated with its inhibition on the COX-2 expression.

关闭