补肾调经方对输卵管性不孕症患者体外受精—胚胎移植超排卵周期GDF-9的影响

Effect of Bushen Tiaojing Recipe on Growth Differentiation Factor-9 in Tubal Infertility Patients Undergoing in vitro Fertilization-Embryo Transfer

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DOI：

中文关键词: 补肾调经方体外受精—胚胎移植生长分化因子-9 卵泡液颗粒细胞

英文关键词:Bushen Tiaojing Recipe in vitro fertilization-embryo transfer growth differentiation factor-9 the follicular fluid granulosa cell

基金项目:

作者	单位
朱爱萍	白求恩国际和平医院妇产科生殖中心
张敏	白求恩国际和平医院妇产科生殖中心
张建平	白求恩国际和平医院妇产科生殖中心
高星	河北医科大学中西医结合学院妇科教研室
杜惠兰	河北医科大学中西医结合学院妇科教研室
常秀峰	河北医科大学中西医结合学院妇科教研室

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中文摘要:

目的研究补肾调经方对提高体外受精—胚胎移植(in vitrofertilization-embryo transfer,IVF-ET)超排卵周期所获卵和胚胎质量的作用及其机制。方法将58例因输卵管性不孕行IVF-ET患者随机分为中药+控制性超排卵组(治疗组,30例)和单纯控制性超排卵组(对照组,28例)。应用Western blot法测定取卵日成熟卵泡卵泡液中生长分化因子-9(growth differentiation factor-9,GDF-9)的含量;用RT-PCR法检测颗粒细胞中GDF-9的表达;并比较两组的促性腺激素(gonadotropin,Gn)用药量、获卵数、受精率、卵裂率、优质胚胎率及妊娠率等。结果治疗组卵泡液中GDF-9水平及颗粒细胞中GDF-9的表达均显著高于对照组(P<0.05);治疗组的获卵数、受精率、优质胚胎率及妊娠率均显著高于对照组(P<0.05);Gn用量及卵裂率两组比较差异无统计学意义。结论补肾调经方可以提高IVF-ET妊娠率,其作用机制可能与调控卵泡液和颗粒细胞GDF-9水平有关。

英文摘要:

Objective To study the effect and mechanism of Bushen Tiaojing Recipe on improving oocyte and embryo qualities in patients undergoing in vitro fertilization-embryo transfer(IVF-ET) at the super-ovulatory cycle.Methods Fifty-eight tubal infertility patients undergoing IVF-ET were randomly assigned to two groups.Thirty patients in the treatment group were treated with Bushen Tiaojing Recipe and GnRHa/FSH/hCG,and twenty-eight patients in the control group were treated with GnRHa/FSH/hCG.Contents of GDF-9 in the mature follicular fluid were detected by Western blot.The expressions of GDF-9 in granulose cells were detected by Real-time PCR.The dose of gonadotropin(Gn),the number of oocytes obtained,the fertilization rate,the oocyte cleavage rate,the high quality embryo rate,and the pregnancy rate were compared.Results The contents of GDF-9 in the follicular fluid and its expression in granulosa cells were significantly higher in the treatment group than in the control group(P<0.05).The number of oocytes obtained,the fertilization rate,the high quality embryo rate,and the pregnancy rate were significantly higher in the treatment group than in the control group.There was no significant difference in the dose of Gn or the oocyte cleavage rate.Conclusions Bushen Tiaojing Recipe could improve the pregnancy rate of IVF-ET.Its mechanism might be possibly through regulating the GDF-9 contents in the follicular fluid and granulosa cells.

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