半夏白术天麻汤对自发性高血压大鼠血管内皮功能的影响

作者	单位
王勋	上海中医药大学教学实验中心
王现珍	上海中医药大学教学实验中心
罗珊珊	上海中医药大学教学实验中心
陆家凤	上海中医药大学教学实验中心
可燕	美国德克萨斯大学休斯顿医学院综合生物及药理学系德克萨斯大学分子医学研究所
蒋嘉烨	上海中医药大学教学实验中心
卞卡	上海高校一氧化氮与炎症医学研究院

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中文摘要:

目的探讨半夏白术天麻汤(Banxia Baizhu Tianma Decoction,BBTD)对自发性高血压大鼠(spontaneous hypertensive rats,SHR)高血压发展进程中的血管内皮功能修复作用及其机制。方法 54只SHR随机分为3组:空白组、半夏白术天麻汤组和卡托普利组,7~24周给予半夏白术天麻汤[生药4.32g/(kg.d)]和卡托普利[3.375g/(kg.d)],停药观察至32周。另取18只对应周龄的WKY大鼠作为空白对照组。18、24和32周时分别采用颈动脉插管测定大鼠血压值,离体血管环检测肠系膜上动脉乙酰胆碱(ACh)浓度依赖性舒张功能;Griess和FRAP法分别测定血清NO2-水平和总抗氧化能力;实时定量荧光PCR(Real-timePCR)检测动脉组织IL-1、IL-6及iNOS mRNA的表达。结果半夏白术天麻汤组在18、24时均可显著降低SHR的血压及血清中NO2-浓度(P<0.05),在18周龄时,增加SHR的总抗氧化能力(P<0.01),在24周龄时显著增加SHR肠系膜上动脉的舒张功能,在18、24和32周龄时,显著降低IL-1 mRNA的表达(P<0.05),在32周龄时显著降低IL-6及iNOS mRNA表达(P<0.01)。卡托普利在18和24周龄时均可显著降低SHR的动脉压(P<0.05),在18周龄时,显著增加SHR血清的总抗氧化能力(P<0.01),但在18周龄、24周龄和32周龄时都不能增加SHR肠系膜上动脉的舒张功能和调节NO2-浓度,在24和32周龄时显著降低iNOSmRNA表达(P<0.01),在32周龄时降低IL-1及IL-6 mRNA的表达(P<0.01)。结论半夏白术天麻汤在降血压方面与卡托普利类似,但在改善SHR肠系膜上动脉内皮功能方面效果显著,该作用可能与其抑制iN-OS和IL-1表达,改善血管微环境的氧化应激状态有关。

英文摘要:

Objective To study the vascular endothelial function recovery and its mechanism of Banxia Baizhu Tianma Decoction(BBTD).Methods 54 SH rats were randomly divided into three groups: the blank control group,BBTD group and Captopril treatment group.BBTD(at the daily dose of 4.320 g crude drug/kg) and Captopril(at the daily dose of 3.375 g/kg) was administered from the 7th week to the 24th week.Another eighteen Wistar-Kyoto rats of the same ages were taken as the control.Medication was discontinued and effects were observed until the 32nd week.The blood pressure was determined by arterial carotis cannula.The concentration of serum NO2-and total anti-oxidation were determined by Griess and fluorescence recovery after photobleaching(FRAP).The acetylcholine(Ach)-dependent relaxation of superior mesenteric artery was detected using in vitro vascular ring.The mRNA expressions of IL-1,IL-6 and iNOS were detected by Real-time PCR at the 18th,24th,and 32nd week.Results BBTD could significantly lower blood pressure of SHR and the concentration of serum NO2-at the 18th and 24th week(P<0.05).The total anti-oxidation of SH rats increased at the 18th week(P<0.01),and ACh-dependent relaxation of superior mesenteric artery increased at the 24th week.The mRNA expressions of IL-1 was markedly suppressed by BBTD at the 18th,24th,and 32nd week(P<0.05),while IL-6 and iNOS mRNA expression were significantly lowered only at the 32nd week(P<0.01).Captopril could significantly lower blood pressure of SHR at the 18th and 24th week(P<0.05).It significantly increased the total anti-oxidation of SH rats at the 18th week(P<0.01).However,it could not increase ACh-dependent relaxation of superior mesenteric artery and regulate the concentration of NO2-at the 18th,24th,and 32nd week.The mRNA expression of iNOS was markedly suppressed by Captopril at the 24th and 32nd week,while mRNA expressions of IL-1 and IL-6 were significantly lower only at the 32nd week(P<0.01).Conclusions BBTD showed similar effect in decreasing the blood pressure to captopril,but it showed better effect in improving the mesenteric endothelial dysfunction of SHR,which may be associated with its inhibition on NO and IL-1 expression,and improvement of the oxidative stress state.

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