复方贞术调脂胶囊对动脉粥样硬化兔脂质过氧化及内皮功能的影响

作者	单位
姚红霞	广东药学院中药药理教研室
杨超燕	广东药学院中药药理教研室
韦燕萍	广东药学院中药药理教研室
王亮	广东药学院中药药理教研室
唐春萍	广东药学院中药药理教研室
江涛	广东药学院实验动物中心
郭姣	国家中医药管理局高脂血症/调肝降脂重点研究室国家中医药管理局脂代谢三级实验室
陈芳超	广东药学院中药药理教研室

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中文摘要:

目的观察复方贞术调脂胶囊(Modified Zhenzhu Tiaozhi Capsule,MZTC)抗兔动脉粥样硬化(atherosclerosis,AS)的作用并探讨其可能机制。方法取50只家兔分为正常组、模型组、辛伐他汀组(3 mg/kg)、MZTC高剂量组(1.6 g生药/kg)、MZTC低剂量组(0.8 g生药/kg),每组10只。采用高脂饮食法建立兔AS模型,药物组在高脂饮食的基础上每天给予相应的药物,连续喂养12周。检测血脂水平、血清超氧化物歧化酶(SOD)、丙二醛(MDA)和NO水平,测定主动脉内膜斑块面积比,观察主动脉病理形态学变化。结果 MZTC组血清TC、TG、LDL-C、MDA水平及动脉粥样硬化指数(AI)较模型组显著降低(P<0.05,P<0.01),HDL-C、SOD和NO水平明显升高(P<0.05,P<0.01);但MZTC组的TC、TG、LDL-C水平和AI明显高于辛伐他汀组(P<0.01),其他指标差异无统计学意义。各用药组主动脉内膜斑块面积占内膜总面积比均显著低于模型组(P<0.01)。光镜观察结果显示,各给药组主动脉病变程度均明显减轻。结论 MZ-TC能抑制AS斑块的形成和发展,其作用机制可能与其调节脂质代谢、抗脂质过氧化和改善内皮功能有关。

英文摘要:

Objective To observe the anti-atherosclerotic effect and its possible mechanisms of Modified Zhenzhu Tiaozhi Capsule(MZTC) in rabbits.Methods 50 rabbits were divided into five groups,i.e.,the normal group,the model group,the simvastatin group(3 mg/kg),the high dose MZTC group(1.6 g crude drug/kg),and the low dose MZTC group(1.6 g crude drug/kg),respectively,ten in each group.The atherosclerosis(AS) model was established by feeding rabbits with high fat diet.Corresponding medicines were administrated on the basis of high fat diet for twelve successive weeks.Levels of blood lipids,serum superoxide dismutase(SOD),malondialdehyde(MDA),and nitric oxide(NO) were detected.The area of the aortic tunica intima plaque was determined.The pathological changes of the aorta were observed.Results Compared with the model group,serum levels of total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),MDA,and atherosclerotic index(AI) were significantly lowered in the MZTC groups(P<0.05,P<0.01),while serum levels of high-density lipoprotein cholesterol(HDL-C),SOD,and NO obviously increased(P<0.05,P<0.01).Serum levels of TC,TG,LDL-C,and in the MZTC groups were obviously higher than those of the simvastatin group(P<0.01),and there was insignificant difference in other indices.The ratio of the aortic tunica intima plaque percentage to the total tunica intima area was also markedly lower than that of the model group(P<0.05,P<0.01).Results under light microscope indicated the pathological changes of the aorta was obviously attenuated.Conclusions MZTC could inhibit the formation and development of AS plaque.Its mechanism might be associated with regulating lipids metabolism,antioxidation,and improving endothelial functions.

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